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Phloem

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Johannes Liesche

    1. College of Life Sciences, Northwest A&F University, Yangling, China

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2014)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Investigating Phloem Anatomy

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Optimal Preparation of Tissue Sections for Light-Microscopic Analysis of Phloem Anatomy

      • Marcelo Rodrigo Pace
      Pages 3-16

    3. Transmission Electron Microscopy of the Phloem with Minimal Artifacts

      • Pascal Hunziker, Alexander Schulz
      Pages 17-27

    4. Scanning Electron Microscopy of the Phloem

      • Daniel L. Mullendore
      Pages 29-35

    5. Noninvasive Investigation of Phloem Structure by 3D Synchrotron X-Ray Microtomography

      • Jussi-Petteri Suuronen, Tuula Jyske
      Pages 37-54

    6. Quantification of Leaf Phloem Anatomical Features with Microscopy

      • Jared J. Stewart, Onno Muller, Christopher M. Cohu, Barbara Demmig-Adams, William W. Adams III
      Pages 55-72

    7. Methods of Phloem Visualization: A Clear Future in Sight?

      • Elisabeth Truernit
      Pages 73-79

  3. Visualizing Phloem Proteins

    1. Front Matter

      Pages 81-81
      Download chapter PDF

    2. Super-Resolution Microscopy of Phloem Proteins

      • Ryan C. Stanfield, Alexander Schulz
      Pages 83-94

    3. Live-Cell Imaging of Fluorescently Tagged Phloem Proteins with Confocal Microscopy

      • Thibaud Cayla, Rozenn Le Hir, Sylvie Dinant
      Pages 95-108

    4. Immunohistochemical Localization of Proteins in the Phloem: Problems and Solutions

      • Christina Kühn
      Pages 109-122

  4. Sampling Phloem Sap

    1. Front Matter

      Pages 123-123
      Download chapter PDF

    2. Studying Phloem Loading with EDTA-Facilitated Phloem Exudate Collection and Analysis

      • Qiyu Xu, Yunjuan Ren, Johannes Liesche
      Pages 125-133

    3. Measurement of Inner Bark and Leaf Osmolality

      • Teemu Paljakka, Anna Lintunen, Yann Salmon, Teemu Hölttä
      Pages 135-142

  5. Measurement of Phloem Transport

    1. Front Matter

      Pages 143-143
      Download chapter PDF

    2. Using 13C to Quantify Phloem Transport on Tall Plants in the Field

      • Daniel Epron, Masako Dannoura, Caroline Plain
      Pages 145-151

    3. Noninvasive Determination of Phloem Transport Speed with Carbon-14 (14C)

      • Christopher Vincent, Peter E. H. Minchin, Johannes Liesche
      Pages 153-162

    4. Detecting Rapid Changes in Carbon Transport and Partitioning with Carbon-11 (11C)

      • Benjamin A. Babst, Richard Ferrieri, Michael Schueller
      Pages 163-176

    5. In Vivo Veritas: Radiotracers in Studies of Phloem Transport of Carbohydrate

      • Michael R. Thorpe, Peter E. H. Minchin
      Pages 177-194
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Keywords

About this book

This detailed book presents methods that enable the determination of parameters relevant to phloem research in the most efficient ways, putting plant scientists with access to adequate instrumentation in the position to answer any phloem-related question. The collection explores techniques that have been used for decades, such as tracing phloem transport with carbon isotopes, as well as recent developments, such as esculin-based assays of phloem transport and super-resolution microscopy of phloem proteins. As such, the book presents the state-of-the-art in phloem research and, at the same time, a starting point for the development of new methods that will fill the remaining gaps in the phloem researcher’s toolbox in the future. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and comprehensive, Phloem: Methods and Protocols is an ideal guide for all researchers exploring this challenging area of plant biology.

Editors and Affiliations

  • College of Life Sciences, Northwest A&F University, Yangling, China

    Johannes Liesche

Bibliographic Information

  • Book Title: Phloem

  • Book Subtitle: Methods and Protocols

  • Editors: Johannes Liesche

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9562-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9561-5Published: 14 June 2019

  • Softcover ISBN: 978-1-4939-9564-6Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9562-2Published: 13 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 473

  • Number of Illustrations: 20 b/w illustrations, 87 illustrations in colour

  • Topics: Plant Sciences

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