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Citrus Tristeza Virus

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Antonino F. Catara

    1. Department of Phytosanitary Sciences, University of Catania, Science and Technology Park of Sicily, Catania, Italy

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  2. Moshe Bar-Joseph

    1. The S. Talkowski Laboratory, Department of Plant Pathology and Weed Research, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel

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  3. Grazia Licciardello

    1. Consiglio per la Ricerca in agricoltura e l’analisi dell’Economia Agraria, Centro di Olivicoltura, Frutticoltura e Agrumicoltura (CREA-OFA), Acireale (Catania), Italy

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2015)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. A Short Note on Reflections and Publications on Citrus tristeza virus (CTV) Methodologies

    • Moshe Bar-Joseph
    Pages 1-6

  3. A Brief Historical Account of the Family Closteroviridae

    • Giovanni P. Martelli
    Pages 7-13

  4. Phenotyping Biological Properties of CTV Isolates

    • Marcella Russo, Antonino F. Catara
    Pages 15-27

  5. CTV Vectors and Interactions with the Virus and Host Plants

    • Raymond Yokomi
    Pages 29-53

  6. Tissue-Print and Squash Capture Real-Time RT-PCR Method for Direct Detection of Citrus tristeza virus (CTV) in Plant or Vector Tissues

    • Mariano Cambra, Eduardo Vidal, Carmen Martínez, Edson Bertolini
    Pages 55-66

  7. Detection of Citrus tristeza virus and Coinfecting Viroids

    • Maria Saponari, Stefania Zicca, Giuliana Loconsole, Beatriz Navarro, Francesco Di Serio
    Pages 67-78

  8. Assessment of Genetic Variability of Citrus tristeza virus by SSCP and CE-SSCP

    • Elisavet K. Chatzivassiliou, Grazia Licciardello
    Pages 79-104

  9. Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus tristeza virus

    • Maria Saponari, Annalisa Giampetruzzi, Vijayanandraj Selvaraj, Yogita Maheshwari, Raymond Yokomi
    Pages 105-126

  10. Genotyping Citrus tristeza virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-on-Chip Device

    • Giuseppe Scuderi, Antonino F. Catara, Grazia Licciardello
    Pages 127-142

  11. Rapid and Sensitive Detection of Citrus tristeza virus Using Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay

    • Dilip Kumar Ghosh, Ashish Warghane, Kajal Kumar Biswas
    Pages 143-150

  12. Amplification and Cloning of Large cDNA Fragments of the Citrus tristeza virus Genome

    • Munir Mawassi, Sabrina Haviv, Ludmila Maslenin
    Pages 151-161

  13. Bioinformatic Tools and Genome Analysis of Citrus tristeza virus

    • Ana Belén Ruiz-García, Rachelle Bester, Antonio Olmos, Hans Jacob Maree
    Pages 163-178

  14. Analysis of Genotype Composition of Citrus tristeza virus Populations Using Illumina Miseq Technology

    • David A. Read, Gerhard Pietersen
    Pages 179-194

  15. Citrus tristeza virus: Host RNA Silencing and Virus Counteraction

    • Susana Ruiz-Ruiz, Beatriz Navarro, Leandro Peña, Luis Navarro, Pedro Moreno, Francesco Di Serio et al.
    Pages 195-207

  16. Proteomic Response of Host Plants to Citrus tristeza virus

    • Milena Santos Dória, Carlos Priminho Pirovani
    Pages 209-218

  17. Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment

    • Yosvanis Acanda, Chunxia Wang, Amit Levy
    Pages 219-228

  18. Methods for Producing Transgenic Plants Resistant to CTV

    • Nuria Soler, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores et al.
    Pages 229-243

  19. Back Matter

    Pages 245-247
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Keywords

About this book

This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.

Editors and Affiliations

  • Department of Phytosanitary Sciences, University of Catania, Science and Technology Park of Sicily, Catania, Italy

    Antonino F. Catara

  • The S. Talkowski Laboratory, Department of Plant Pathology and Weed Research, The Volcani Center, Agricultural Research Organization, Bet Dagan, Israel

    Moshe Bar-Joseph

  • Consiglio per la Ricerca in agricoltura e l’analisi dell’Economia Agraria, Centro di Olivicoltura, Frutticoltura e Agrumicoltura (CREA-OFA), Acireale (Catania), Italy

    Grazia Licciardello

Bibliographic Information

  • Book Title: Citrus Tristeza Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Antonino F. Catara, Moshe Bar-Joseph, Grazia Licciardello

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9558-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9557-8Published: 25 June 2019

  • Softcover ISBN: 978-1-4939-9560-8Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9558-5Published: 20 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 247

  • Number of Illustrations: 14 b/w illustrations, 36 illustrations in colour

  • Topics: Plant Pathology, Virology, Plant Genetics and Genomics

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