Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2012)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (18 protocols)
-
Front Matter
-
Back Matter
About this book
This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions.
Editors and Affiliations
-
EnzyPep B.V., Geleen, The Netherlands
Timo Nuijens, Marcel Schmidt
Bibliographic Information
Book Title: Enzyme-Mediated Ligation Methods
Editors: Timo Nuijens, Marcel Schmidt
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9546-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9545-5Published: 03 June 2019
Softcover ISBN: 978-1-4939-9548-6Published: 14 August 2020
eBook ISBN: 978-1-4939-9546-2Published: 03 June 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 371
Number of Illustrations: 35 b/w illustrations, 59 illustrations in colour
Topics: Biotechnology, Protein-Ligand Interactions, Enzymology