Pathogenic Yersinia

1. Front Matter

   Pages i-xi

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2. Mouse Models for Yersinia Infection

   1. Front Matter

      Pages 1-1

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   2. An Intradermal Model for Yersinia pestis Inoculation

      • Rodrigo J. Gonzalez

      Pages 3-15

   3. Intranasal Inoculation of Mice with Yersinia pestis and Processing of Pulmonary Tissue for Analysis

      • Roger D. Pechous

      Pages 17-28

   4. Standardized Method for Aerosol Challenge of Rodents with Yersinia pestis for Modeling Primary Pneumonic Plague

      • Paul E. Anderson, Rachel M. Olson, Joshua L. Willix, Deborah M. Anderson

      Pages 29-39

   5. Mouse Models of Yersiniosis

      • Diana Hooker-Romero, Leah Schwiesow, Yahan Wei, Victoria Auerbuch

      Pages 41-53

3. Monitoring Bacteria During Infection

   1. Front Matter

      Pages 55-55

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   2. Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-Transmitted Yersinia pestis

      • Jeffrey G. Shannon, B. Joseph Hinnebusch

      Pages 57-68

   3. Laser Scanning Microscopy of Yersinia pestis Infected Tissues

      • Rodrigo J. Gonzalez

      Pages 69-84

   4. Real-Time Monitoring of Yersinia pestis Promoter Activity by Bioluminescence Imaging

      • Anne Derbise, Olivier Dussurget, Elisabeth Carniel, Javier Pizarro-Cerdá

      Pages 85-97

   5. Discovering Yersinia–Host Interactions by Tissue Dual RNA-Seq

      • Maria Kusmierek, Ann Kathrin Heroven, Michael Beckstette, Aaron M. Nuss, Petra Dersch

      Pages 99-116

   6. Detection of Cells Translocated with Yersinia Yops in Infected Tissues Using β-Lactamase Fusions

      • Giang T. Nguyen, Anne L. McCabe, Alyssa C. Fasciano, Joan Mecsas

      Pages 117-139

   7. Monitoring of Neutrophil Recruitment to Mice Lungs During Pneumonic Plague

      • Yaron Vagima, Yinon Levy, Emanuelle Mamroud

      Pages 141-150

4. Invertebrate Models of Infection (In Vivo and In Vitro)

   1. Front Matter

      Pages 151-151

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   2. Co-infection Assay to Determine Yersinia pestis Competitive Fitness in Fleas

      • Athena Lemon, Amelia Silva-Rohwer, Janelle Sagawa, Viveka Vadyvaloo

      Pages 153-166

   3. Drosophila as a Model for Understanding the Insect Host of Yersinia pestis

      • Anna B. Ludlow, Cassandra D. Pauling, Melanie M. Marketon, Deborah M. Anderson

      Pages 167-178

5. Yersinia Interaction with Immune Cells and Immune Signaling

   1. Front Matter

      Pages 179-179

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   2. Intracellular Assays to Monitor Survival and Growth of Yersinia pestis Within Macrophages

      • Amanda R. Pulsifer, Tiva T. VanCleave, Matthew B. Lawrenz

      Pages 181-196

   3. Human Neutrophil Isolation and Degranulation Responses to Yersinia pestis Infection

      • Kara R. Eichelberger, William E. Goldman

      Pages 197-209

   4. Measurement of Yersinia Translocon Pore Formation in Erythrocytes

      • Tiago R. D. Costa, Monika K. Francis, Salah I. Farag, Tomas Edgren, Matthew S. Francis

      Pages 211-229

This volume delivers a compendium of detailed protocols to the research community in order to aid in the investigation and discovery of Yersinia virulence mechanisms using important in vivo and in vitro infection models, which have led to major advances in the field and in our understanding of Yersinia pathogen-host interactions. Beginning with a section on mouse models, the book continues with chapters covering the monitoring of bacteria during infection, invertebrate models, as well as Yersinia interaction with immune cells and immune signaling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and authoritative, Pathogenic Yersinia: Methods and Protocols providesa single source for researchers seeking to better understand these pathogens and the diseases they produce in humans.

• Virulence mechanisms
• Infection models
• Host-pathogen interactions
• Mouse models
• Bacterial proliferation
• Immune response
• infectious diseases

“The book is written for the research community, including novices. Clinicians and parasitologists with an interest in investigation and discovery of Yersinia virulence mechanisms may also find this book helpful and interesting. … This is a wonderful resource for understanding the investigation and discovery of the virulence mechanisms of Yersinia. … This book is quite unique in covering the methods and protocols related to Yersinia pathogen‐host interactions.” (Omer Iqbal, Doody's Book Reviews, November 01, 2019)

• Paul G Allen School for Global Animal Health, Washington State University, Pullman, USA

  Viveka Vadyvaloo

• Department of Microbiolgy and Immunology and Center for Predictive Medicine in Biodefense and Emerging Infectious Diseases, University of Louisville School of Medicine, Louisville, USA

  Matthew B. Lawrenz

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