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Stem Cell Niche

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Hospital Research Institute, Ottawa, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2002)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres

    • Sofie Mohlin, Laura Kerosuo
    Pages 1-11

  3. Analysis of Hematopoietic Niche in the Mouse Embryo

    • Keai Sinn Tan, Nathalie Brouard, Daisuke Sugiyama
    Pages 13-27

  4. Functional Assays of Stem Cell Properties Derived from Different Niches

    • Beatriz de Lucas, Laura M. Pérez, Beatriz G. Gálvez
    Pages 29-38

  5. Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche

    • Aviva J. Goel, Robert S. Krauss
    Pages 39-50

  6. Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment

    • Madeline Campbell, Mamta Chabria, Gemma A. Figtree, Liudmila Polonchuk, Carmine Gentile
    Pages 51-59

  7. Isolation, Propagation, and Clonogenicity of Intestinal Stem Cells

    • Prashanthi Ramesh, Aleksandar Buryanov Kirov, David Johannes Huels, Jan Paul Medema
    Pages 61-73

  8. Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells

    • Mary C. Morton, Victoria N. Neckles, David M. Feliciano
    Pages 75-85

  9. Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering

    • Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao, Yan Jin
    Pages 87-99

  10. Three-Dimensional Co-culture of Human Hematopoietic Stem/Progenitor Cells and Mesenchymal Stem/Stromal Cells in a Biomimetic Hematopoietic Niche Microenvironment

    • Marta H. G. Costa, Tiago S. Monteiro, Susana Cardoso, Joaquim M. S. Cabral, Frederico Castelo Ferreira, Cláudia L. da Silva
    Pages 101-119

  11. Investigating the Vascular Niche: Three-Dimensional Co-culture of Human Skeletal Muscle Stem Cells and Endothelial Cells

    • Claire Latroche, Michèle Weiss-Gayet, Bénédicte Chazaud
    Pages 121-128

  12. Identification and Characterization of Stem Cells in Oral Cancer

    • Sujit K. Bhutia, Prajna P. Naik, Prakash P. Praharaj, Debasna P. Panigrahi, Chandra S. Bhol, Kewal K. Mahapatra et al.
    Pages 129-139

  13. Determining Competitive Potential of Bone Metastatic Cancer Cells in the Murine Hematopoietic Stem Cell Niche

    • Sun H. Park, Matthew R. Eber, Russell S. Taichman, Yusuke Shiozawa
    Pages 141-150

  14. Spatial Genomic Analysis: A Multiplexed Transcriptional Profiling Method that Reveals Subpopulations of Cells Within Intact Tissues

    • Antti Lignell, Laura Kerosuo
    Pages 151-163

  15. 3D Culture of Mesenchymal Stem Cells in Alginate Hydrogels

    • Sílvia J. Bidarra, Cristina C. Barrias
    Pages 165-180

  16. Isolation and Identification of Murine Bone Marrow-Derived Macrophages and Osteomacs from Neonatal and Adult Mice

    • Joydeep Ghosh, Safa F. Mohamad, Edward F. Srour
    Pages 181-193

  17. Back Matter

    Pages 195-199
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Keywords

About this book

This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the first edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Niche: Methods and Protocols, Second Edition serves as an ideal guide for both experts and novices in the stem cell field.

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Stem Cell Niche

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9508-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9507-3Published: 07 June 2019

  • Softcover ISBN: 978-1-4939-9510-3Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9508-0Published: 06 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 199

  • Number of Illustrations: 6 b/w illustrations, 29 illustrations in colour

  • Topics: Stem Cells, Cell Physiology

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