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Neisseria gonorrhoeae

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Myron Christodoulides

    1. Molecular Microbiology Group, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1997)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Biology of the Gonococcus: Disease and Pathogenesis

    • Jutamas Shaughnessy, Sanjay Ram, Peter A. Rice
    Pages 1-27

  3. How to Identify Exposed Women Who Are Infected with Neisseria gonorrhoeae

    • Stephanie E. McLaughlin, J. McLeod Griffiss
    Pages 29-36

  4. Antimicrobial Resistance in Neisseria gonorrhoeae and Treatment of Gonorrhea

    • Magnus Unemo, Daniel Golparian, David W. Eyre
    Pages 37-58

  5. Prediction of Minimum Inhibitory Concentrations of Antimicrobials for Neisseria gonorrhoeae Using Whole-Genome Sequencing

    • David W. Eyre, Daniel Golparian, Magnus Unemo
    Pages 59-76

  6. Gonococcal Colony Typing

    • John E. Heckels
    Pages 77-85

  7. Preparation of Lipooligosaccharide (LOS) from Neisseria gonorrhoeae

    • Myron Christodoulides
    Pages 87-96

  8. Purification of Type IV Pili and Pilin Subunits

    • Lisa Craig, Tuba Altindal
    Pages 97-110

  9. Peptidoglycan Composition in Neisseria

    • Ryan E. Schaub, Joseph P. Dillard
    Pages 111-120

  10. Isolation of Naturally Released Gonococcal Outer Membrane Vesicles as Vaccine Antigens

    • Ian P. Francis, Xiuping Lui, Lee M. Wetzler
    Pages 121-141

  11. Transformation in Neisseria gonorrhoeae

    • Melanie M. Callaghan, Joseph P. Dillard
    Pages 143-162

  12. Strategies for Global RNA Sequencing of the Human Pathogen Neisseria gonorrhoeae

    • Ryan McClure, Caroline A. Genco
    Pages 163-183

  13. Bioinformatics Workflow for Gonococcal Proteomics

    • Fadi E. El-Rami, Aleksandra E. Sikora
    Pages 185-205

  14. Phenotypic MicroArray Screening of Neisseria gonorrhoeae in Chemically Defined Liquid Medium

    • Benjamin I. Baarda, Aleksandra E. Sikora
    Pages 207-216

  15. Generation of Metal-Depleted Conditions for In Vitro Growth of Neisseria gonorrhoeae

    • Cynthia Nau Cornelissen
    Pages 217-231

  16. Cloning, Expression, and Purification of Recombinant Neisseria gonorrhoeae Proteins

    • María Victoria Humbert
    Pages 233-266

  17. Complement-Dependent Serum Bactericidal Assays for Neisseria gonorrhoeae

    • Sunita Gulati, Peter A. Rice, Sanjay Ram
    Pages 267-280

  18. Basic Methods for Examining Neisseria gonorrhoeae Interactions with Host Cells In Vitro

    • Hannia Liliana Almonacid-Mendoza, Myron Christodoulides
    Pages 281-299

  19. Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection

    • Jimena Gatica, Paula I. Rodas, Alejandro Escobar
    Pages 301-318

  20. Protocols to Interrogate the Interactions Between Neisseria gonorrhoeae and Primary Human Neutrophils

    • Stephanie A. Ragland, Alison K. Criss
    Pages 319-345
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Keywords

About this book

This detailed book addresses the continual development and application of new methods and protocols to understand the biology of the pathogen Neisseria gonorrhoeae. Beginning with gonococcus biology and antimicrobial resistance, the volume continues with areas of study such as producing major gonococcal antigens to high purity, genetic transformation of gonococci and strategies for global RNA sequencing, evaluation of potential vaccine/drug targets and the assessment of hypothetical protein function, as well as a number of methods and protocols for studying the biological interactions of the gonococcus and the host. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and cutting-edge, Neisseria gonorrhoeae: Methods and Protocols provides researchers with a foundation for progress toward the eventual control of this disease and a significant reduction in global case numbers of gonorrhea.

Editors and Affiliations

  • Molecular Microbiology Group, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK

    Myron Christodoulides

Bibliographic Information

  • Book Title: Neisseria gonorrhoeae

  • Book Subtitle: Methods and Protocols

  • Editors: Myron Christodoulides

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9496-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9495-3Published: 23 May 2019

  • Softcover ISBN: 978-1-4939-9498-4Published: 12 August 2020

  • eBook ISBN: 978-1-4939-9496-0Published: 22 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 461

  • Number of Illustrations: 10 b/w illustrations, 51 illustrations in colour

  • Topics: Bacteriology, Infectious Diseases, Drug Resistance, Vaccine

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