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Metabolomics

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Sanjoy K. Bhattacharya

    1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1996)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer

    • Lukáš Najdekr, Giovanny Rodriguez Blanco, Warwick B. Dunn
    Pages 1-15

  3. Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis

    • Chris Beecher, Felice A. de Jong
    Pages 17-28

  4. Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals

    • Mukundan Ragavan, Matthew E. Merritt
    Pages 29-40

  5. Quantitative Metabolomics Using Isotope Residue Outlier Analysis (IROA®) with Internal Standards

    • Roberto Mendez, Maria del Carmen Piqueras, Alexander Raskind, Felice A. de Jong, Chris Beecher, Sanjoy K. Bhattacharya et al.
    Pages 41-46

  6. Analyses of Cholesterol Metabolites of Optic Nerve Using GC-MS Methods

    • Gurkaran S. Sarohia, Erik D. Garza, Sanjoy K. Bhattacharya
    Pages 47-51

  7. Analyses of Cholesterol and Derivatives in Ocular Tissues Using LC-MS/MS Methods

    • Maria Piqueras, Despoina Theotoka, Gurkaran S. Sarohia, Sanjoy K. Bhattacharya
    Pages 53-59

  8. Measurement of Pyridine Nucleotides in Biological Samples Using LC-MS/MS

    • Chris Petucci, Jeffrey A. Culver, Nidhi Kapoor, E. Hampton Sessions, Daniela Divlianska, Stephen J. Gardell
    Pages 61-73

  9. Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates

    • Anita H. Lewin, Peter Silinski
    Pages 75-94

  10. Protein–Lipid Complex Separation Utilizing a Capillary Electrophoresis System

    • Anddre Osmar Valdivia, Ciara Myer, Maria Fernanda Suarez, Sanjoy K. Bhattacharya
    Pages 95-100

  11. Immature and Mature Collagen Crosslinks Quantification Using High-Performance Liquid Chromatography and High-Resolution Mass Spectrometry in Orbitrap™

    • Diana R. Hernandez, Maria del Carmen Piqueras, Alejandro E. Macias, Laisel Martinez, Roberto Vazquez-Padron, Sanjoy K. Bhattacharya
    Pages 101-111

  12. Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS)

    • Pallavi Lahiri, Deepika Dhaware, Ajeet Singh, Venkateswarlu Panchagnula, Dipankar Ghosh
    Pages 113-129

  13. Analysis of Microbial Siderophores by Mass Spectrometry

    • Tomas Pluhacek, Anton Skriba, Jiri Novak, Dominika Luptakova, Vladimir Havlicek
    Pages 131-153

  14. HSD18B7 Enzyme Assay Technique Using a Triple Quadrupole Mass Spectrometer

    • Carina Wu, Nhuong-Sao Ton, Maria Fernanda Suarez, Maria Piqueras, Sanjoy K. Bhattacharya
    Pages 155-159

  15. Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification

    • Chiranjit Maity, Dipankar Ghosh, Sonia Guha
    Pages 161-178

  16. Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites

    • Maria Del Carmen Piqueras, Ciara Myer, Anna Junk, Sanjoy K. Bhattacharya
    Pages 179-185

  17. Assessment of Transport of Lipid Metabolites Within Trabecular Meshwork Cells

    • Daphne Toglia, Stephanie Dancausse, Karthik Kalahasty, Bryan Alfonso, Sanjoy K. Bhattacharya
    Pages 187-197

  18. Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes

    • Shamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra Merscher, Alessia Fornoni, Hassan Al-Ali
    Pages 199-206

  19. Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis

    • Kousik Kesh, Sulagna Banerjee
    Pages 207-216

  20. Metabolomics Analyses from Tissues in Parkinson’s Disease

    • Fatema Bhinderwala, Shulei Lei, Jade Woods, Jordan Rose, Darrell D. Marshall, Eli Riekeberg et al.
    Pages 217-257
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Keywords

About this book

The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson’s Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls.

Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested inthis exciting and evolving field. 



Editors and Affiliations

  • Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, USA

    Sanjoy K. Bhattacharya

Bibliographic Information

  • Book Title: Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Sanjoy K. Bhattacharya

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9488-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9487-8Published: 25 May 2019

  • Softcover ISBN: 978-1-4939-9490-8Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9488-5Published: 24 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 335

  • Number of Illustrations: 29 b/w illustrations, 53 illustrations in colour

  • Topics: Metabolomics, Mass Spectrometry

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