Skip to main content
SpringerLink
Log in
Book cover

Skin Tissue Engineering

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Sophie Böttcher-Haberzeth

    1. University Children’s Hospital Zurich, Zurich, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Thomas Biedermann

    1. University Children’s Hospital Zurich, Zurich, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1993)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Isolation and Culture of Skin Cells

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Isolation and Culture of Human Keratinocytes

      • Sergio Cortez Ghio, Gaëtan Le-Bel, Amélie Lavoie, Danielle Larouche, Lucie Germain
      Pages 3-13

    3. Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture

      • Daisuke Suzuki, Filipa Pinto, Makoto Senoo
      Pages 15-31

    4. Isolation and Culture of Epidermal Melanocytes

      • Muriel Cario, Alain Taieb
      Pages 33-46

    5. Long-Term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-β Signaling Inhibitor

      • Filipa Pinto, Daisuke Suzuki, Makoto Senoo
      Pages 47-59

    6. Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells

      • Dongrui Ma, Seng-Teik Lee, Alvin Wen Choong Chua
      Pages 61-70

    7. Isolation and Culture of Human Dermal Fibroblasts

      • Marta A. Kisiel, Agnes S. Klar
      Pages 71-78

    8. Isolation and Culture of Human Dermal Microvascular Endothelial Cells

      • Jennifer Bourland, Dominique Mayrand, Nathalie Tremblay, Véronique J. Moulin, Julie Fradette, François A. Auger
      Pages 79-90

    9. Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue

      • Joris A. van Dongen, Martin C. Harmsen, Hieronymus P. Stevens
      Pages 91-103

  3. Tissue Engineering Skin and Engineered Skin Models

    1. Front Matter

      Pages 105-105
      Download chapter PDF

    2. Engineering a Multilayered Skin Equivalent: The Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility

      • Lydia Costello, Nicola Fullard, Mathilde Roger, Steven Bradbury, Teresa Dicolandrea, Robert Isfort et al.
      Pages 107-122

    3. Three-Dimensional Epidermal Model from Human Hair Follicle-Derived Keratinocytes

      • Takamitsu Matsuzawa, Michiyo Nakano, Ayako Oikawa, Yuumi Nakamura, Hiroyuki Matsue
      Pages 123-137

    4. Fabrication of a Co-Culture System with Human Sweat Gland-Derived Cells and Peripheral Nerve Cells

      • Matthias Brandenburger, Charli Kruse
      Pages 139-148

    5. Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose-Derived Stem Cells, and Adipocytes

      • Maike Keck, Alfred Gugerell, Johanna Kober
      Pages 149-157

    6. Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering

      • Andrea L. Lalley, Steven T. Boyce
      Pages 159-179

    7. Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models

      • Yinghong He, Cristina Has
      Pages 181-190

  4. In Vitro and Vivo Models Testing Skin, Skin Cells, and Tissue Engineered Skin

    1. Front Matter

      Pages 191-191
      Download chapter PDF

    2. Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts

      • Regine C. Tölle, Jörn Dengjel
      Pages 193-204

    3. Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy

      • Gery Barmettler, Urs Ziegler
      Pages 205-215
Back to top

Keywords

About this book

This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.

Editors and Affiliations

  • University Children’s Hospital Zurich, Zurich, Switzerland

    Sophie Böttcher-Haberzeth, Thomas Biedermann

Bibliographic Information

  • Book Title: Skin Tissue Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Sophie Böttcher-Haberzeth, Thomas Biedermann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9473-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9472-4Published: 31 May 2019

  • Softcover ISBN: 978-1-4939-9475-5Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9473-1Published: 30 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 263

  • Number of Illustrations: 13 b/w illustrations, 66 illustrations in colour

  • Topics: Regenerative Medicine/Tissue Engineering, Cell Culture

Publish with us

Policies and ethics

Back to top

Search

Navigation