Plant Cell Morphogenesis

1. Front Matter

   Pages i-xi

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2. Essential Methods of Plant Sample Preparation for Light Microscopy

   • Aleš Soukup, Edita Tylová

   Pages 1-26

3. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy

   • Aleš Soukup

   Pages 27-42

4. Chemical Fixation, Immunofluorescence, and Immunogold Labeling of Electron Microscopical Sections

   • Ilse Foissner, Margit Hoeftberger

   Pages 43-62

5. Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature

   • Jana Nebesářová

   Pages 63-76

6. Fluorescence Lifetime Imaging of Plant Cell Walls

   • Christine Terryn, Gabriel Paës

   Pages 77-82

7. Raman Spectroscopy in Nonwoody Plants

   • Dorota Borowska-Wykręt, Mateusz Dulski

   Pages 83-107

8. Image Analysis: Basic Procedures for Description of Plant Structures

   • Jana Albrechtová, Zuzana Kubínová, Aleš Soukup, Jiří Janáček

   Pages 109-119

9. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing

   • Fatima Cvrčková

   Pages 121-133

10. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics

    • Amparo Rosero, Denisa Oulehlová, Viktor Žárský, Fatima Cvrčková

    Pages 135-149

11. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D

    • Birgit Möller, Luise Zergiebel, Katharina Bürstenbinder

    Pages 151-171

12. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions

    • Adriana Jelínková, Kateřina Malínská, Jan Petrášek

    Pages 173-187

13. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells

    • Lise C. Noack, Přemysl Pejchar, Juraj Sekereš, Yvon Jaillais, Martin Potocký

    Pages 189-199

14. The Photoconvertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics

    • Alexandra Lešková, Zuzana Kusá, Mária Labajová, Miroslav Krausko, Ján Jásik

    Pages 201-214

15. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells

    • Mateusz Majda, Aleksandra Sapala, Anne-Lise Routier-Kierzkowska, Richard S. Smith

    Pages 215-230

16. Optical Trapping in Plant Cells

    • Tijs Ketelaar, Norbert de Ruijter, Stefan Niehren

    Pages 231-238

17. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation

    • Dorota Kwiatkowska, Sandra Natonik-Białoń, Agata Burian

    Pages 239-255

18. Time-Lapse Imaging of Developing Shoot Meristems Using A Confocal Laser Scanning Microscope

    • Olivier Hamant, Pradeep Das, Agata Burian

    Pages 257-268

19. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX

    • Soeren Strauss, Aleksandra Sapala, Daniel Kierzkowski, Richard S. Smith

    Pages 269-290

20. Kinematic Characterization of Root Growth by Means of Stripflow

    • Tobias I. Baskin, Ellen Zelinsky

    Pages 291-305

This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs.

• Cell wall
• Plant cell structures
• Intracellular structures
• Model cell types
• In vivo imaging

• Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

  Fatima Cvrčková, Viktor Žárský

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