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Redox-Mediated Signal Transduction

Methods and Protocols

  • Book
  • © 2019

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1990)

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Table of contents (15 protocols)

Keywords

About this book

This volume explores the scope of the cellular redox analysis and the importance of not being limited by frequently changing and evolving technology. The chapters in this book cover a wide range of topics such as redox components in animal and plant cells and the role of reactive oxygen species, reactive nitrogen species, and hydrogen sulphide in cell signaling; measuring modifications using Flow Cytometry, ELISA assays, and Western blot analysis; measurement of oxidative stress in mitochondira and biological systems; and the use of the genetically encoded fluorescent probe HyPer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 
Cutting-edge and informative, Redox-Mediated Signal Transduction: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in the evolving redox field. 


Editors and Affiliations

  • Department of Applied Sciences, University of the West of England, Bristol, UK

    John T. Hancock, Myra E. Conway

Bibliographic Information

  • Book Title: Redox-Mediated Signal Transduction

  • Book Subtitle: Methods and Protocols

  • Editors: John T. Hancock, Myra E. Conway

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9463-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9461-8Published: 31 May 2019

  • eBook ISBN: 978-1-4939-9463-2Published: 30 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 199

  • Number of Illustrations: 19 b/w illustrations, 15 illustrations in colour

  • Topics: Cell Biology

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