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Plant Innate Immunity

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1991)

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Hardcover Book USD 169.99
Price excludes VAT (USA)
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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
  2. Quantification of Extracellular ATP in Plant Suspension Cell Cultures

    • Sowmya R. Ramachandran, Sonika Kumar, Kiwamu Tanaka
    Pages 43-54
  3. Method to Study Dynamics of Membrane-Bound Plant Transcription Factors During Biotic Interactions in Tomato

    • Supriyo Chowdhury, Payel Bhattacharjee, Shrabani Basak, Shreya Chowdhury, Pallob Kundu
    Pages 61-68
  4. Preparation of Plant Material for Analysis of Protein–Nucleic Acid Interactions by FRET-FLIM

    • Maxime Escouboué, Laurent Camborde, Alain Jauneau, Elodie Gaulin, Laurent Deslandes
    Pages 69-77
  5. Analysis of DNA Methylation Profile in Plants by Chop-PCR

    • Pratiti Dasgupta, Shubho Chaudhuri
    Pages 79-90
  6. Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry

    • Leiyun Yang, Zhixue Wang, Jian Hua
    Pages 101-106
  7. A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem Under Various Light Environments

    • Daniel L. Leuchtman, Anthony D. Shumate, Walter Gassmann, Emmanuel Liscum
    Pages 107-113
  8. Methods for Replicating Leaf Vibrations Induced by Insect Herbivores

    • Sabrina C. J. Michael, Heidi A. Appel, Reginald B. Cocroft
    Pages 141-157
  9. The Apple Fruitlet Model System for Fire Blight Disease

    • Sara M. Klee, Judith P. Sinn, Timothy W. McNellis
    Pages 187-198

About this book

The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource forall researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.


Editors and Affiliations

  • Division of Plant Sciences, Christopher S. Bond Life Sciences Center, and Interdisciplinary Plant Group, University of Missouri, Columbia, USA

    Walter Gassmann

Bibliographic Information

  • Book Title: Plant Innate Immunity

  • Book Subtitle: Methods and Protocols

  • Editors: Walter Gassmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9458-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9457-1Published: 30 April 2019

  • eBook ISBN: 978-1-4939-9458-8Published: 30 April 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 260

  • Number of Illustrations: 17 b/w illustrations, 42 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access