Cell Culture Techniques

1. Front Matter

   Pages i-xiii

   PDF

2. In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model

   • Emma H. Neal, Yajuan Shi, Ethan S. Lippmann

   Pages 1-15

3. In Vitro Techniques for Assessing Neurotoxicity Using Human iPSC-Derived Neuronal Models

   • Anke M. Tukker, Fiona M. J. Wijnolts, Aart de Groot, Richard W. Wubbolts, Remco H. S. Westerink

   Pages 17-35

4. Oxidative Stress Signatures in Human Stem Cell-Derived Neurons

   • M. Diana Neely, Aaron B. Bowman

   Pages 37-49

5. Glial Reactivity in Response to Neurotoxins: Relevance and Methods

   • Lindsay T. Michalovicz, James P. O’Callaghan

   Pages 51-67

6. Neuron-Glia Interactions Studied with In Vitro Co-Cultures

   • S. Mancino, M. M. Serafini, Barbara Viviani

   Pages 69-85

7. Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria

   • Monica Rodriguez-Silva, Kristen T. Ashourian, Anthony D. Smith, Jeremy W. Chambers

   Pages 87-118

8. Epigenetic Changes in Cultures: Neurons and Astrocytes

   • David P. Gavin, Xiaolu Zhang, Marina Guizzetti

   Pages 119-139

9. The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation

   • Laura Nimtz, Jördis Klose, Stefan Masjosthusmann, Marta Barenys, Ellen Fritsche

   Pages 141-168

10. Zebrafish as a Tool to Assess Developmental Neurotoxicity

    • Keturah G. Kiper, Jennifer L. Freeman

    Pages 169-193

11. Rat Brain Slices: An Optimum Biological Preparation for Acute Neurotoxicological Studies

    • Gabriela Aguilera-Portillo, Aline Colonnello-Montero, Marisol Maya-López, Edgar Rangel-López, Abel Santamaría

    Pages 195-207

12. Electrophysiological Neuromethodologies

    • Yukun Yuan, William D. Atchison

    Pages 209-231

13. A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies

    • Zhen He, John Panos, James Raymick, Tetyana Konak, Li Cui, Diane B. Miller et al.

    Pages 233-243

14. Transporter Studies: Brain Punching Technique

    • Cherish A. Taylor, Somshuvra Mukhopadhyay

    Pages 245-253

15. miRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders

    • Lena Smirnova, Alexandra Maertens

    Pages 255-281

16. Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes

    • Anna Bal-Price, Francesca Pistollato

    Pages 283-299

17. Back Matter

    Pages 301-302

    PDF

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

 
Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.

• Neurotoxicity
• in vitro
• iPSCs
• GCCP
• Neural Stem Cells
• Cell culture

• Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA

  Michael Aschner

• Department of Environment/STE 100, University of Washington, Seattle, USA

  Lucio Costa

Policies and ethics