Skip to main content
SpringerLink
Log in

Cell Culture Techniques

  • Book
  • © 2019

Overview

Editors:
  1. Michael Aschner

    1. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Lucio Costa

    1. Department of Environment/STE 100, University of Washington, Seattle, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 145)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (15 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. In Vitro Blood-Brain Barrier Functional Assays in a Human iPSC-Based Model

    • Emma H. Neal, Yajuan Shi, Ethan S. Lippmann
    Pages 1-15

  3. In Vitro Techniques for Assessing Neurotoxicity Using Human iPSC-Derived Neuronal Models

    • Anke M. Tukker, Fiona M. J. Wijnolts, Aart de Groot, Richard W. Wubbolts, Remco H. S. Westerink
    Pages 17-35

  4. Oxidative Stress Signatures in Human Stem Cell-Derived Neurons

    • M. Diana Neely, Aaron B. Bowman
    Pages 37-49

  5. Glial Reactivity in Response to Neurotoxins: Relevance and Methods

    • Lindsay T. Michalovicz, James P. O’Callaghan
    Pages 51-67

  6. Neuron-Glia Interactions Studied with In Vitro Co-Cultures

    • S. Mancino, M. M. Serafini, Barbara Viviani
    Pages 69-85

  7. Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Stress-Responsive Proteins to Mitochondria

    • Monica Rodriguez-Silva, Kristen T. Ashourian, Anthony D. Smith, Jeremy W. Chambers
    Pages 87-118

  8. Epigenetic Changes in Cultures: Neurons and Astrocytes

    • David P. Gavin, Xiaolu Zhang, Marina Guizzetti
    Pages 119-139

  9. The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation

    • Laura Nimtz, Jördis Klose, Stefan Masjosthusmann, Marta Barenys, Ellen Fritsche
    Pages 141-168

  10. Zebrafish as a Tool to Assess Developmental Neurotoxicity

    • Keturah G. Kiper, Jennifer L. Freeman
    Pages 169-193

  11. Rat Brain Slices: An Optimum Biological Preparation for Acute Neurotoxicological Studies

    • Gabriela Aguilera-Portillo, Aline Colonnello-Montero, Marisol Maya-López, Edgar Rangel-López, Abel Santamaría
    Pages 195-207

  12. Electrophysiological Neuromethodologies

    • Yukun Yuan, William D. Atchison
    Pages 209-231

  13. A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies

    • Zhen He, John Panos, James Raymick, Tetyana Konak, Li Cui, Diane B. Miller et al.
    Pages 233-243

  14. Transporter Studies: Brain Punching Technique

    • Cherish A. Taylor, Somshuvra Mukhopadhyay
    Pages 245-253

  15. miRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders

    • Lena Smirnova, Alexandra Maertens
    Pages 255-281

  16. Application of Non-Animal Methods to More Effective Neurotoxicity Testing for Regulatory Purposes

    • Anna Bal-Price, Francesca Pistollato
    Pages 283-299

  17. Back Matter

    Pages 301-302
    Download chapter PDF
Back to top

Keywords

About this book

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

 
Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.


Editors and Affiliations

  • Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA

    Michael Aschner

  • Department of Environment/STE 100, University of Washington, Seattle, USA

    Lucio Costa

Bibliographic Information

  • Book Title: Cell Culture Techniques

  • Editors: Michael Aschner, Lucio Costa

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-9228-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9227-0Published: 26 April 2019

  • Softcover ISBN: 978-1-4939-9230-0Published: 02 October 2020

  • eBook ISBN: 978-1-4939-9228-7Published: 25 April 2019

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 2

  • Number of Pages: XIII, 302

  • Number of Illustrations: 10 b/w illustrations, 47 illustrations in colour

  • Topics: Neurosciences, Cell Culture

Publish with us

Policies and ethics

Back to top

Search

Navigation