Neisseria meningitidis

1. Front Matter

   Pages i-xiii

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2. An Overview of Neisseria meningitidis

   • Sarah Hollingshead, Christoph M. Tang

   Pages 1-16

3. Diagnosis of Meningococcal Infection Using Internally Controlled Multiplex Real-Time PCR

   • Ala-Eddine Deghmane, Eva Hong, Muhamed-Kheir Taha

   Pages 17-31

4. Investigating RNA–Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis

   • Nadja Heidrich, Saskia Bauriedl, Christoph Schoen

   Pages 33-49

5. Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics

   • Holly B. Bratcher, Odile B. Harrison, Martin C. J. Maiden

   Pages 51-82

6. Determination of Repeat Number and Expression States of Phase-Variable Loci Through Next Generation Sequencing and Bioinformatic Analysis

   • Luke R. Green, Richard D. Haigh, Christopher D. Bayliss

   Pages 83-92

7. Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis

   • Freda E. -C. Jen, Kate L. Seib, Aimee Tan

   Pages 93-104

8. Sample Preparation for Proteomic Analysis of Neisseria meningitidis

   • Benjamin L. Schulz

   Pages 105-112

9. Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance

   • Tsitsi D. Mubaiwa, Lauren E. Hartley-Tassell, Evgeny A. Semchenko, Christopher J. Day, Michael P. Jennings, Kate L. Seib

   Pages 113-121

10. The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp.

    • Emily A. Kibble, Mitali Sarkar-Tyson, Geoffrey W. Coombs, Charlene M. Kahler

    Pages 123-133

11. In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells

    • Brandon J. Kim, Alexandra Schubert-Unkmeir

    Pages 135-148

12. Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease

    • Kay O. Johswich, Scott D. Gray-Owen

    Pages 149-168

13. Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays

    • Jay Lucidarme, Jennifer Louth, Kelly Townsend-Payne, Ray Borrow

    Pages 169-179

14. Monoclonal Antibodies Against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitidis: A Platform for the Quality Control of Meningococcal Vaccines

    • Elizabeth González, Fátima Reyes, Oscar Otero, Frank Camacho, Maribel Cuello, Fidel Ramírez et al.

    Pages 181-203

15. Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine

    • Giuseppe Boccadifuoco, Brunella Brunelli, Elena Mori, Mauro Agnusdei, Claudia Gianfaldoni, Marzia Monica Giuliani

    Pages 205-215

16. Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitidis

    • Jakob Loschko, Karen Garcia, David Cooper, Michael Pride, Annaliesa Anderson

    Pages 217-236

17. Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis

    • Caroline Cayrou, Christopher D. Bayliss

    Pages 237-249

18. Back Matter

    Pages 251-253

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This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration andconsistency in investigations of this enigmatic and dangerous pathogen.

• Meningococcus
• Strain analysis
• Vaccine development
• Disease progression
• Neisseria gonorrhoeae
• Host cells
• vaccines
• infectious diseases

• Institute For Glycomics, Griffith University, Southport, Australia

  Kate L. Seib, Ian R. Peak

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