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Nuclear Receptors

Methods and Experimental Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Mostafa Z. Badr

    1. School of Pharmacy, University of Missouri-Kansas City, Kansas City, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1966)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Nuclear Receptors: A Historical Perspective

    • Gisela I. Mazaira, Nadia R. Zgajnar, Cecilia M. Lotufo, Cristina Daneri-Becerra, Jeffrey C. Sivils, Olga B. Soto et al.
    Pages 1-5

  3. An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues

    • Thomas W. Gettys, Ji Suk Chang
    Pages 7-16

  4. Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue

    • Aisling Smyth, Martina Gogarty, Daniel Crean, Evelyn P. Murphy
    Pages 17-26

  5. In Vivo Quantitation of Estrogen Receptor β Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy

    • Linda S. M. Gulliver
    Pages 27-38

  6. In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High-Confidence Genome-Wide Binding Profiles

    • Ashfaq Ali Mir, Kenneth Allen Dyar, Franziska Greulich, Fabiana Quagliarini, Céline Jouffe, Michaël Jean Hubert et al.
    Pages 39-70

  7. High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation

    • Bryan Mackowiak, Hongbing Wang
    Pages 71-77

  8. Antibody Validation Strategy for Nuclear Receptors

    • Borbala Katona, Ahmed Ibrahim, MÃ¥rten Sundberg, Cecilia Williams
    Pages 79-99

  9. Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed, Paraffin-Embedded Tissue

    • Elizabeth D. Lightbody, Christopher J. B. Nicol
    Pages 101-105

  10. Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus

    • Teddy Kamata, Chun-Song Yang, Kasey Jividen, Adam Spencer, Natalia Dworak, Luke T. Oostdyk et al.
    Pages 107-124

  11. Reconstitution of the Steroid Receptor Heterocomplex

    • Gisela I. Mazaira, Mario D. Galigniana
    Pages 125-135

  12. High-Throughput Imaging of PPIX Using Confocal Microscopy

    • C. Trent Brewer, Jonathan Low, Taosheng Chen
    Pages 137-149

  13. PGC-1α Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle

    • Dongwook Yeo, Chounghun Kang, Li Li Ji
    Pages 151-161

  14. A Reverse Transfection Method for Screening of Nuclear Receptor Activators

    • Jenni Küblbeck, Paavo Honkakoski
    Pages 163-173

  15. Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators

    • Jan Heering, Daniel Merk
    Pages 175-192

  16. Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists

    • Yukimasa Takeda, Hong Soon Kang, Anton M. Jetten
    Pages 193-202

  17. Examining the Role of Nuclear Receptors During In Vivo Chemical-Mediated Breast Tumorigenesis

    • Jia Yue Shi, Rachel E. Rubino, Christopher J. B. Nicol
    Pages 203-210

  18. Analysis of IL-4/STAT6 Signaling in Macrophages

    • Syed F. Hassnain Waqas, Grace Ampem, Tamás Röszer
    Pages 211-224

  19. Isolation and Characterization of Adipose Tissue Macrophages

    • Grace Ampem, Tamás Röszer
    Pages 225-236

  20. Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph

    • Qinqiang Long, Lizhen Huang, Kai Huang, Qinglin Yang
    Pages 237-246
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Keywords

About this book

This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering ourunderstanding of these vital receptors and their role in numerous pathological conditions.

Editors and Affiliations

  • School of Pharmacy, University of Missouri-Kansas City, Kansas City, USA

    Mostafa Z. Badr

Bibliographic Information

  • Book Title: Nuclear Receptors

  • Book Subtitle: Methods and Experimental Protocols

  • Editors: Mostafa Z. Badr

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9195-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9194-5Published: 01 May 2019

  • eBook ISBN: 978-1-4939-9195-2Published: 30 April 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 314

  • Number of Illustrations: 15 b/w illustrations, 57 illustrations in colour

  • Topics: Protein-Ligand Interactions, Protein Science, Pharmacology/Toxicology

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