Nuclear Receptors

1. Front Matter

   Pages i-xii

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2. Nuclear Receptors: A Historical Perspective

   • Gisela I. Mazaira, Nadia R. Zgajnar, Cecilia M. Lotufo, Cristina Daneri-Becerra, Jeffrey C. Sivils, Olga B. Soto et al.

   Pages 1-5

3. An Optimized Immunoblotting Protocol for Accurate Detection of Endogenous PGC-1α Isoforms in Various Rodent Tissues

   • Thomas W. Gettys, Ji Suk Chang

   Pages 7-16

4. Subcellular Localization of NR4A2 Orphan Nuclear Receptor Expression in Human and Mouse Synovial Joint Tissue

   • Aisling Smyth, Martina Gogarty, Daniel Crean, Evelyn P. Murphy

   Pages 17-26

5. In Vivo Quantitation of Estrogen Receptor β Subtype Expression in Ovarian Surface Epithelium Using Immunofluorescence Profiling and Confocal Microscopy

   • Linda S. M. Gulliver

   Pages 27-38

6. In Vivo ChIP-Seq of Nuclear Receptors: A Rough Guide to Transform Frozen Tissues into High-Confidence Genome-Wide Binding Profiles

   • Ashfaq Ali Mir, Kenneth Allen Dyar, Franziska Greulich, Fabiana Quagliarini, Céline Jouffe, Michaël Jean Hubert et al.

   Pages 39-70

7. High-Content Analysis of Constitutive Androstane Receptor Nuclear Translocation

   • Bryan Mackowiak, Hongbing Wang

   Pages 71-77

8. Antibody Validation Strategy for Nuclear Receptors

   • Borbala Katona, Ahmed Ibrahim, Mårten Sundberg, Cecilia Williams

   Pages 79-99

9. Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed, Paraffin-Embedded Tissue

   • Elizabeth D. Lightbody, Christopher J. B. Nicol

   Pages 101-105

10. Detection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus

    • Teddy Kamata, Chun-Song Yang, Kasey Jividen, Adam Spencer, Natalia Dworak, Luke T. Oostdyk et al.

    Pages 107-124

11. Reconstitution of the Steroid Receptor Heterocomplex

    • Gisela I. Mazaira, Mario D. Galigniana

    Pages 125-135

12. High-Throughput Imaging of PPIX Using Confocal Microscopy

    • C. Trent Brewer, Jonathan Low, Taosheng Chen

    Pages 137-149

13. PGC-1α Overexpression via Local In Vivo Transfection in Mouse Skeletal Muscle

    • Dongwook Yeo, Chounghun Kang, Li Li Ji

    Pages 151-161

14. A Reverse Transfection Method for Screening of Nuclear Receptor Activators

    • Jenni Küblbeck, Paavo Honkakoski

    Pages 163-173

15. Hybrid Reporter Gene Assays: Versatile In Vitro Tools to Characterize Nuclear Receptor Modulators

    • Jan Heering, Daniel Merk

    Pages 175-192

16. Analysis of the Transcriptional Activity of Retinoic Acid-Related Orphan Receptors (RORs) and Inhibition by Inverse Agonists

    • Yukimasa Takeda, Hong Soon Kang, Anton M. Jetten

    Pages 193-202

17. Examining the Role of Nuclear Receptors During In Vivo Chemical-Mediated Breast Tumorigenesis

    • Jia Yue Shi, Rachel E. Rubino, Christopher J. B. Nicol

    Pages 203-210

18. Analysis of IL-4/STAT6 Signaling in Macrophages

    • Syed F. Hassnain Waqas, Grace Ampem, Tamás Röszer

    Pages 211-224

19. Isolation and Characterization of Adipose Tissue Macrophages

    • Grace Ampem, Tamás Röszer

    Pages 225-236

20. Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph

    • Qinqiang Long, Lizhen Huang, Kai Huang, Qinglin Yang

    Pages 237-246

This volume presents valuable techniques for studying the class of ligand-activated transcription factors known as nuclear receptors. After a brief overview of the history of the field, chapters cover methods to detect the receptors and their mRNAs in various tissues, protocols to characterize nuclear receptor modulators and activities, their signaling and roles in certain pathogenesis, molecular modeling of nuclear receptor-ligand interactions, as well as the utility of informatics in the field of nuclear receptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Nuclear Receptors: Methods and Experimental Protocols seeks to aid researchers working toward furthering ourunderstanding of these vital receptors and their role in numerous pathological conditions.

• Drug discovery
• NR ligands
• Ligand-activated transcription factors
• Receptor signaling
• Molecular modeling
• Modulators
• Drug development
• Protein-Ligand Interactions

• School of Pharmacy, University of Missouri-Kansas City, Kansas City, USA

  Mostafa Z. Badr

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