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Developmental Toxicology

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Jason M. Hansen

    1. Department of Physiology and Developmental Biology, Brigham Young University, Provo, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Louise M. Winn

    1. Biomedical and Molecular Sciences, Queen’s University, Kingston, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge, fully updated techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1965)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. In Vitro Models in Developmental Toxicology

    • Louise M. Winn
    Pages 1-6

  3. Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments

    • Jason M. Hansen, Ted B. Piorczynski
    Pages 7-17

  4. Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology

    • Charles A. Easley
    Pages 19-34

  5. Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model

    • Mika Suga, Miho K. Furue
    Pages 35-48

  6. Micromass Methods for the Evaluation of Developmental Toxicants

    • Omar J. Mohammed, Margaret K. Pratten
    Pages 49-72

  7. The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System

    • France-HĂ©lène Paradis, Han Yan, Chunwei Huang, Barbara F. Hales
    Pages 73-91

  8. Embryonic Midfacial Palatal Organ Culture Methods in Developmental Toxicology

    • Barbara D. Abbott
    Pages 93-105

  9. Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology

    • Jone A. Stanley, Joe A. Arosh, Patricia B. Hoyer, Sakhila K. Banu
    Pages 107-127

  10. Hepatotoxicity in Zebrafish Larvae

    • Kathryn Bambino, Joshua Morrison, Jaime Chu
    Pages 129-138

  11. Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model

    • Joslynn G. Affleck, Virginia K. Walker
    Pages 139-153

  12. The Chicken Embryo as a Model in Developmental Toxicology

    • Michael R. Stark, Micah M. Ross
    Pages 155-171

  13. The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity

    • Cecilia Berg
    Pages 173-186

  14. Mouse Whole Embryo Culture

    • Emily W. Y. Tung, Louise M. Winn
    Pages 187-194

  15. Rat Whole Embryo Culture

    • Craig Harris
    Pages 195-217

  16. Rabbit Whole Embryo Culture

    • Terence R. S. Ozolinš
    Pages 219-233

  17. Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology

    • Brendon K. Warner, Jonathan K. Alder, Arminda Suli
    Pages 235-250

  18. Identifying Toxicant-Interacting Genes Using Forward Genetic Screening in Zebrafish

    • Jonathon T. Hill
    Pages 251-259

  19. Assessment of Histiotrophic Nutrition Using Fluorescent Probes

    • Craig Harris
    Pages 261-279

  20. Examining Gene Expression Patterns Through Whole-Mount In Situ Hybridization

    • Jeffery R. Barrow
    Pages 281-295
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Keywords

About this book

This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab. 

Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Editionserves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures.

Editors and Affiliations

  • Department of Physiology and Developmental Biology, Brigham Young University, Provo, USA

    Jason M. Hansen

  • Biomedical and Molecular Sciences, Queen’s University, Kingston, Canada

    Louise M. Winn

Bibliographic Information

  • Book Title: Developmental Toxicology

  • Book Subtitle: Methods and Protocols

  • Editors: Jason M. Hansen, Louise M. Winn

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9182-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9181-5Published: 09 May 2019

  • eBook ISBN: 978-1-4939-9182-2Published: 08 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 436

  • Number of Illustrations: 51 b/w illustrations, 104 illustrations in colour

  • Topics: Pharmacology/Toxicology, Embryology, Cell Biology

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