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Lymphoma

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Ralf Küppers

    1. Institute of Cell Biology (Cancer Research), Medical School, University of Duisburg-Essen, Nordrhein-Westfalen, Essen, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1956)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Origin and Pathogenesis of B Cell Lymphomas

    • Marc Seifert, René Scholtysik, Ralf Küppers
    Pages 1-33

  3. Flow Cytometry for Non-Hodgkin and Hodgkin Lymphomas

    • Emily Glynn, Lori Soma, David Wu, Brent L. Wood, Jonathan R. Fromm
    Pages 35-60

  4. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells

    • Ralf Küppers, Markus Schneider, Martin-Leo Hansmann
    Pages 61-75

  5. PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations

    • Elke Boone, Kim C. Heezen, Patricia J. T. A. Groenen, Anton W. Langerak, On behalf of the EuroClonality Consortium
    Pages 77-103

  6. Expression Cloning of Antibodies from Single Human B Cells

    • Hedda Wardemann, Christian E. Busse
    Pages 105-125

  7. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ-)PCR

    • Menno C. van Zelm
    Pages 127-138

  8. Stereotyped B Cell Receptor Immunoglobulins in B Cell Lymphomas

    • Andreas Agathangelidis, Fotis Psomopoulos, Kostas Stamatopoulos
    Pages 139-155

  9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

    • Sebastian Böttcher
    Pages 157-197

  10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

    • Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H. J. van der Velden, Jacques J. M. van Dongen, Michael Kneba
    Pages 199-228

  11. Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input

    • Dieter Weichenhan, Charles D. Imbusch, Qi Wang, Benedikt Brors, Christoph Plass
    Pages 229-248

  12. FISH and FICTION in Lymphoma Research

    • Maciej Giefing, Reiner Siebert
    Pages 249-267

  13. NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma

    • Joost Kluiver, Fubiao Niu, Ye Yuan, Klaas Kok, Anke van den Berg, Agnieszka Dzikiewicz-Krawczyk
    Pages 269-282

  14. RNA Sequencing in B-Cell Lymphomas

    • Da Wei Huang, Moez Dawood, Calvin A. Johnson, Roland Schmitz
    Pages 283-303

  15. Studying Cancer Heterogeneity by Single-Cell RNA Sequencing

    • Johannes W. Bagnoli, Lucas E. Wange, Aleksandar Janjic, Wolfgang Enard
    Pages 305-319

  16. Evaluation of Whole Genome Sequencing Data

    • Daniel Hübschmann, Matthias Schlesner
    Pages 321-336

  17. Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines

    • Daniel E. Webster, Sandrine Roulland, James D. Phelan
    Pages 337-350

  18. Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens

    • Katarzyna Tomska, Sebastian Scheinost, Thorsten Zenz
    Pages 351-362

  19. Proximity Ligation Assay

    • Ryan M. Young
    Pages 363-370

  20. Phosphoproteomic Analysis of Signaling Pathways in Lymphomas

    • Björn Häupl, Henning Urlaub, Thomas Oellerich
    Pages 371-381
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Keywords

About this book

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 



Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology.

Editors and Affiliations

  • Institute of Cell Biology (Cancer Research), Medical School, University of Duisburg-Essen, Nordrhein-Westfalen, Essen, Germany

    Ralf Küppers

Bibliographic Information

  • Book Title: Lymphoma

  • Book Subtitle: Methods and Protocols

  • Editors: Ralf Küppers

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9151-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9150-1Published: 19 February 2019

  • eBook ISBN: 978-1-4939-9151-8Published: 18 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 439

  • Number of Illustrations: 18 b/w illustrations, 48 illustrations in colour

  • Topics: Cancer Research

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