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The Extracellular Matrix

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Davide Vigetti

    1. Department of Medicine and Surgery, University of Insubria, Varese, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Achilleas D. Theocharis

    1. Department of Chemistry, University of Patras, Patras, Greece

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1952)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. The Complex Interplay Between Extracellular Matrix and Cells in Tissues

    • Dimitra Manou, Ilaria Caon, Panagiotis Bouris, Irene-Eva Triantaphyllidou, Cristina Giaroni, Alberto Passi et al.
    Pages 1-20

  3. Method for Studying ECM Expression: In Situ RT-PCR

    • Elena Caravà, Cristiana Marcozzi, Barbara Bartolini, Marcella Reguzzoni, Paola Moretto, Ilaria Caon et al.
    Pages 21-31

  4. Visualizing the Supramolecular Assembly of Collagen

    • Mario Raspanti, Marcella Reguzzoni, Petra Rita Basso, Marina Protasoni, Désirée Martini
    Pages 33-44

  5. Steady-State and Pulse-Chase Analyses of Fibrillar Collagen

    • Antonella Forlino, Francesca Tonelli, Roberta Besio
    Pages 45-53

  6. Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates

    • Mariana P. Stelling, Ananda A. de Bento, Philippe Caloba, Eduardo Vilanova, Mauro S. G. Pavão
    Pages 55-70

  7. Analysis of Proteoglycan Synthesis and Secretion in Cell Culture Systems

    • Chiara Paganini, Rossella Costantini, Antonio Rossi
    Pages 71-80

  8. Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples

    • Sanna Oikari, Markku I. Tammi
    Pages 81-89

  9. Methods for Hyaluronan Molecular Mass Determination by Agarose Gel Electrophoresis

    • Mary K. Cowman
    Pages 91-102

  10. Hyaluronan Pericellular Matrix: Particle Exclusion Assay

    • Melanie A. Simpson
    Pages 103-110

  11. Determination of Cell-Surface Hyaluronan Through Flow Cytometry

    • Daiana L. Vitale, Fiorella M. Spinelli, Laura Alaniz
    Pages 111-116

  12. Method for Detecting Hyaluronan in Isolated Myenteric Plexus Ganglia of Adult Rat Small Intestine

    • Michela Bistoletti, Paola Moretto, Cristina Giaroni
    Pages 117-125

  13. Analyzing Hyaluronidases in Biological Fluids

    • Christos Velesiotis, Stella Vasileiou, Demitrios H. Vynios
    Pages 127-142

  14. Method for Studying the Physical Effect of Extracellular Matrix on Voltage-Dependent Ion Channel Gating

    • Eleonora Solari, Andrea Moriondo
    Pages 143-156

  15. Methods for Monitoring Matrix-Induced Autophagy

    • Carolyn Chen, Aastha Kapoor, Renato V. Iozzo
    Pages 157-191

  16. Method for Determining Gelatinolytic Activity in Tissue: In Situ Gelatin Zymography

    • Elin Hadler-Olsen, Jan-Olof Winberg
    Pages 193-199

  17. Method for Determining Gelatinolytic Activity in Tissue Extracts: Real-Time Gelatin Zymography

    • Elin Hadler-Olsen, Jan-Olof Winberg
    Pages 201-210

  18. In Vitro Spheroid Sprouting Assay of Angiogenesis

    • Fatema Tuz Zahra, Efrossini Choleva, Md Sanaullah Sajib, Evangelia Papadimitriou, Constantinos M. Mikelis
    Pages 211-218

  19. Matrigel Plug Assay for In Vivo Evaluation of Angiogenesis

    • Pinelopi Kastana, Fatema Tuz Zahra, Despoina Ntenekou, Stamatiki Katraki-Pavlou, Dimitris Beis, Michail S. Lionakis et al.
    Pages 219-232

  20. Exosomes from Cell Culture-Conditioned Medium: Isolation by Ultracentrifugation and Characterization

    • Anurag Purushothaman
    Pages 233-244
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Keywords

About this book

This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.

Editors and Affiliations

  • Department of Medicine and Surgery, University of Insubria, Varese, Italy

    Davide Vigetti

  • Department of Chemistry, University of Patras, Patras, Greece

    Achilleas D. Theocharis

Bibliographic Information

  • Book Title: The Extracellular Matrix

  • Book Subtitle: Methods and Protocols

  • Editors: Davide Vigetti, Achilleas D. Theocharis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9133-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9132-7Published: 04 March 2019

  • eBook ISBN: 978-1-4939-9133-4Published: 01 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 278

  • Number of Illustrations: 22 b/w illustrations, 27 illustrations in colour

  • Topics: Cell Biology, Biochemistry, general

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