Skip to main content
SpringerLink
Log in
Book cover

Alpha-Synuclein

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Tim Bartels

    1. Dementia Research Institute, University College London, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1948)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Assays for Neuronal Defects Caused by Early Formation of α-Synuclein Inclusions in Primary Cultured Neurons

    • Laura A. Volpicelli-Daley
    Pages 1-14

  3. Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain Tissue

    • John B. Sanderson
    Pages 15-22

  4. Kinetic Measurements of Endocytosis and Exocytosis in Cultured Neurons

    • Karina J. Vargas
    Pages 23-33

  5. Detection of Misfolded α-Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification Platform

    • Luis Concha-Marambio, Mohammad Shahnawaz, Claudio Soto
    Pages 35-44

  6. Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain Using Preformed Fibrils

    • Bin Zhang, Victoria Kehm, Ron Gathagan, Susan N. Leight, John Q. Trojanowski, Virginia M.-Y. Lee et al.
    Pages 45-57

  7. Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based Detection

    • Niels Kruse, Brit Mollenhauer
    Pages 59-68

  8. Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein Oligomers

    • Rosalind F. Roberts, Nora Bengoa-Vergniory, Javier Alegre-Abarrategui
    Pages 69-76

  9. Studying α-Synuclein Conformation by Intact-Cell Cross-Linking

    • Thibaut Imberdis, Saranna Fanning, Andrew Newman, Nagendran Ramalingam, Ulf Dettmer
    Pages 77-91

  10. C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and Techniques

    • Mandy Koopman, Renée I. Seinstra, Ellen A. A. Nollen
    Pages 93-112

  11. Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein

    • Tara A. Eastwood, Daniel P. Mulvihill
    Pages 113-121

  12. Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of α-Synuclein with Membranes

    • Matteo Rovere
    Pages 123-143

  13. Yeast-Based Screens to Target Alpha-Synuclein Toxicity

    • Inês Caldeira Brás, Blagovesta Popova, Gerhard H. Braus, Tiago F. Outeiro
    Pages 145-156

  14. Probing Structural Changes in Alpha-Synuclein by Nuclear Magnetic Resonance Spectroscopy

    • Tapojyoti Das, David Eliezer
    Pages 157-181

  15. Measuring Mitochondrial Dysfunction Caused by Soluble α-Synuclein Oligomers

    • Eric S. Luth, Irina G. Stavrovskaya
    Pages 183-198

  16. Monitoring α-Synuclein Proteotoxicity in Drosophila Models

    • Aron Szabo, George K. Tofaris
    Pages 199-208

  17. Detection of α-Synuclein in Biological Samples Using Mass Spectrometry

    • Ann Brinkmalm, Annika Öhrfelt, Payel Bhattacharjee, Henrik Zetterberg
    Pages 209-220

  18. Single-Molecule FRET Detection of Early-Stage Conformations in α-Synuclein Aggregation

    • Mahdi Muhammad Moosa, Josephine C. Ferreon, Allan Chris M. Ferreon
    Pages 221-233

  19. In Situ Peroxidase Labeling and Mass Spectrometry of Alpha-Synuclein in Rat Cortical Neurons

    • Ricardo Sanz, Patrick Ovando-Roche, Namrata D. Udeshi, Steven A. Carr, Chee Yeun Chung, Vikram Khurana
    Pages 235-246

  20. Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) Spectroscopy

    • Julia Cattani, Theresa Braun, Malte Drescher
    Pages 247-260
Back to top

Keywords

About this book

This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects.


Editors and Affiliations

  • Dementia Research Institute, University College London, London, UK

    Tim Bartels

Bibliographic Information

  • Book Title: Alpha-Synuclein

  • Book Subtitle: Methods and Protocols

  • Editors: Tim Bartels

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9124-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9123-5Published: 18 February 2019

  • eBook ISBN: 978-1-4939-9124-2Published: 15 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 289

  • Number of Illustrations: 11 b/w illustrations, 55 illustrations in colour

  • Topics: Protein Science, Neurosciences

Publish with us

Policies and ethics

Back to top

Search

Navigation