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Collagen

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Irit Sagi

    1. Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

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    You can also search for this editor in PubMed   Google Scholar

  2. Nikolaos A. Afratis

    1. Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1944)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. In Vitro Models for Collagen Characterization

    1. Front Matter

      Pages 1-1
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    2. Cloning and Mutagenesis Strategies for Large Collagens

      • Olivier Bornert, Alexander Nyström
      Pages 3-15

    3. Multi-well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen

      • Venkat Raghavan Krishnaswamy
      Pages 17-22

    4. Production and Characterization of Chemically Cross-Linked Collagen Scaffolds

      • Ignacio Sallent, Héctor Capella-Monsonís, Dimitrios I. Zeugolis
      Pages 23-38

    5. A Biomimetic Model for Mineralization of Type-I Collagen Fibrils

      • Shasha Yao, Yifei Xu, Changyu Shao, Fabio Nudelman, Nico A. J. M. Sommerdijk, Ruikang Tang
      Pages 39-54

  3. Proteomics Analysis of Collagen

    1. Front Matter

      Pages 55-55
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    2. Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy

      • Wing Ying Chow
      Pages 57-77

    3. Measurement of Collagen Cross-Links from Tissue Samples by Mass Spectrometry

      • Amar Joshi, Amna Zahoor, Alberto Buson
      Pages 79-93

    4. Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes

      • Ngoc-Duc Doan, Andrew S. DiChiara, Amanda M. Del Rosario, Richard P. Schiavoni, Matthew D. Shoulders
      Pages 95-114

    5. Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics

      • Elizabeta Madzharova, Fabio Sabino, Ulrich auf dem Keller
      Pages 115-126

  4. Imaging Approaches for Collagen

    1. Front Matter

      Pages 127-127
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    2. Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy

      • Elee Shimshoni, Irit Sagi
      Pages 129-133

    3. In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology

      • Yang Li, S. Michael Yu
      Pages 135-144

    4. Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics

      • Stéphane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Petr Dokládal, Florence Ruggiero, Jean-Marc Allain et al.
      Pages 145-155

    5. Analysis of Collagen-Binding Integrin Interactions with Supramolecular Aggregates of the Extracellular Matrix

      • Uwe Hansen
      Pages 157-166

  5. Collagen in Health and Disease

    1. Front Matter

      Pages 167-167
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    2. Assessing Collagen Deposition During Aging in Mammalian Tissue and in Caenorhabditis elegans

      • Alina C. Teuscher, Cyril Statzer, Sophia Pantasis, Mattia R. Bordoli, Collin Y. Ewald
      Pages 169-188

    3. Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis

      • Efrat Kessler, Eyal Hassoun
      Pages 189-201

    4. Tumorigenic Interplay Between Macrophages and Collagenous Matrix in the Tumor Microenvironment

      • Chen Varol
      Pages 203-220
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Keywords

About this book

This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.

Editors and Affiliations

  • Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel

    Irit Sagi, Nikolaos A. Afratis

Bibliographic Information

  • Book Title: Collagen

  • Book Subtitle: Methods and Protocols

  • Editors: Irit Sagi, Nikolaos A. Afratis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9095-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9094-8Published: 07 March 2019

  • eBook ISBN: 978-1-4939-9095-5Published: 06 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 254

  • Number of Illustrations: 16 b/w illustrations, 44 illustrations in colour

  • Topics: Protein Science, Cell Biology

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