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Mouse Cell Culture

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Ivan Bertoncello

    1. Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, Australia

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1940)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Practical Considerations

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Applicability of Mouse Models to the Study of Human Disease

      • Kristina Rydell-Törmänen, Jill R. Johnson
      Pages 3-22

    3. Optimizing the Cell Culture Microenvironment

      • Ivan Bertoncello
      Pages 23-30

  3. Methods and Protocols

    1. Front Matter

      Pages 31-31
      Download chapter PDF

    2. Propagation and Maintenance of Mouse Embryonic Stem Cells

      • Jacob M. Paynter, Joseph Chen, Xiaodong Liu, Christian M. Nefzger
      Pages 33-45

    3. Production of High-Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells

      • Michael R. Larcombe, Jan Manent, Joseph Chen, Ketan Mishra, Xiaodong Liu, Christian M. Nefzger
      Pages 47-61

    4. Generation of Mouse-Induced Pluripotent Stem Cells by Lentiviral Transduction

      • Xiaodong Liu, Joseph Chen, Jaber Firas, Jacob M. Paynter, Christian M. Nefzger, Jose M. Polo
      Pages 63-76

    5. Gene Editing of Mouse Embryonic and Epiblast Stem Cells

      • Tennille Sibbritt, Pierre Osteil, Xiaochen Fan, Jane Sun, Nazmus Salehin, Hilary Knowles et al.
      Pages 77-95

    6. Identification of Circulating Endothelial Colony-Forming Cells from Murine Embryonic Peripheral Blood

      • Yang Lin, Chang-Hyun Gil, Mervin C. Yoder
      Pages 97-107

    7. Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labelled Cell Culture

      • Matthew Jones, Saverio Bellusci
      Pages 109-127

    8. Mouse Hematopoietic Stem Cell Modification and Labelling by Transduction and Tracking Posttransplantation

      • Benjamin Cao, Songhui Li, Claire Pritchard, Brenda Williams, Susan K. Nilsson
      Pages 129-142

    9. Genetic Manipulation and Selection of Mouse Mesenchymal Stem Cells for Delivery of Therapeutic Factors In Vivo

      • Donald S. Sakaguchi
      Pages 143-155

    10. Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells

      • Apoorva Mulay, Khondoker Akram, Lynne Bingle, Colin D. Bingle
      Pages 157-168

    11. Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells

      • Helen P. Makarenkova, Robyn Meech
      Pages 169-180

    12. Organotypic Culture of Adult Mouse Retina

      • Brigitte Müller
      Pages 181-191

    13. Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes

      • Matthew Ackers-Johnson, Roger S. Foo
      Pages 193-204

    14. Isolation, Culture, and Characterization of Primary Mouse Epidermal Keratinocytes

      • Ling-Juan Zhang
      Pages 205-215

    15. Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells

      • Julie M. Sheridan, Jane E. Visvader
      Pages 217-229

    16. An Organoid Assay for Long-Term Maintenance and Propagation of Mouse Prostate Luminal Epithelial Progenitors and Cancer Cells

      • Yu Shu, Chee Wai Chua
      Pages 231-254
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Keywords

About this book

This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.

Editors and Affiliations

  • Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, Australia

    Ivan Bertoncello

Bibliographic Information

  • Book Title: Mouse Cell Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Ivan Bertoncello

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9086-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9085-6Published: 20 February 2019

  • eBook ISBN: 978-1-4939-9086-3Published: 20 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 315

  • Number of Illustrations: 9 b/w illustrations, 55 illustrations in colour

  • Topics: Cell Culture, Animal Models

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