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  • © 2019

Mouse Cell Culture

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1940)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. Practical Considerations

    1. Front Matter

      Pages 1-1
    2. The Applicability of Mouse Models to the Study of Human Disease

      • Kristina Rydell-Törmänen, Jill R. Johnson
      Pages 3-22
    3. Optimizing the Cell Culture Microenvironment

      • Ivan Bertoncello
      Pages 23-30
  3. Methods and Protocols

    1. Front Matter

      Pages 31-31
    2. Propagation and Maintenance of Mouse Embryonic Stem Cells

      • Jacob M. Paynter, Joseph Chen, Xiaodong Liu, Christian M. Nefzger
      Pages 33-45
    3. Production of High-Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells

      • Michael R. Larcombe, Jan Manent, Joseph Chen, Ketan Mishra, Xiaodong Liu, Christian M. Nefzger
      Pages 47-61
    4. Generation of Mouse-Induced Pluripotent Stem Cells by Lentiviral Transduction

      • Xiaodong Liu, Joseph Chen, Jaber Firas, Jacob M. Paynter, Christian M. Nefzger, Jose M. Polo
      Pages 63-76
    5. Gene Editing of Mouse Embryonic and Epiblast Stem Cells

      • Tennille Sibbritt, Pierre Osteil, Xiaochen Fan, Jane Sun, Nazmus Salehin, Hilary Knowles et al.
      Pages 77-95
    6. Mouse Hematopoietic Stem Cell Modification and Labelling by Transduction and Tracking Posttransplantation

      • Benjamin Cao, Songhui Li, Claire Pritchard, Brenda Williams, Susan K. Nilsson
      Pages 129-142
    7. Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells

      • Apoorva Mulay, Khondoker Akram, Lynne Bingle, Colin D. Bingle
      Pages 157-168
    8. Organotypic Culture of Adult Mouse Retina

      • Brigitte Müller
      Pages 181-191
    9. Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes

      • Matthew Ackers-Johnson, Roger S. Foo
      Pages 193-204
    10. Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells

      • Julie M. Sheridan, Jane E. Visvader
      Pages 217-229

About this book

This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.

Editors and Affiliations

  • Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, Australia

    Ivan Bertoncello

Bibliographic Information

  • Book Title: Mouse Cell Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Ivan Bertoncello

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9086-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9085-6Published: 20 February 2019

  • eBook ISBN: 978-1-4939-9086-3Published: 20 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 315

  • Number of Illustrations: 9 b/w illustrations, 55 illustrations in colour

  • Topics: Cell Culture, Animal Models

Buy it now

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access