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Glutamate Receptors

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Corinna Burger

    1. Department of Neurology, University of Wisconsin, Madison, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Margaret Jo Velardo

    1. ANSER, Homeland Security Studies and Analysis Institute, Falls Church, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1941)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Synaptic Ultrastructure/Receptor Dynamics/Receptor Interactions and Trafficking

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Single-Molecule FRET Methods to Study Glutamate Receptors

      • Douglas B. Litwin, Ryan J. Durham, Vasanthi Jayaraman
      Pages 3-16

    3. Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM)

      • Ai Takahashi-Nakazato, Laxmi Kumar Parajuli, Hirohide Iwasaki, Shinji Tanaka, Shigeo Okabe
      Pages 17-27

    4. Considerations for Imaging and Analyzing Neural Structures by STED Microscopy

      • Martin O. Lenz, Jan Tønnesen
      Pages 29-46

    5. Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors

      • Elek Molnár
      Pages 47-54

  3. Function/Cellular Plasticity

    1. Front Matter

      Pages 55-55
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    2. Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function

      • Andres Barria
      Pages 57-64

    3. Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE)

      • Nathanael O’Neill, Sergiy Sylantyev
      Pages 65-78

    4. Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus

      • Regina U. Hegemann, Wickliffe C. Abraham
      Pages 79-91

    5. Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats

      • Kirstan E. Gimse, Kenneth O’Riordan, Corinna Burger
      Pages 93-105

    6. Whole-Cell Patch-Clamp Electrophysiology to Study Ionotropic Glutamatergic Receptors and Their Roles in Addiction

      • Jonna M. Leyrer-Jackson, M. Foster Olive, Cassandra D. Gipson
      Pages 107-135

  4. Receptor Gene Regulation/Epigenetics

    1. Front Matter

      Pages 137-137
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    2. Gene Expression Analysis by Multiplex Single-Cell RT-PCR

      • Ludovic Tricoire, Bruno Cauli, Bertrand Lambolez
      Pages 139-154

    3. Metabolomics Analysis of Glutamate Receptor Function

      • Nataliya E. Chorna, Anatoliy P. Chornyy
      Pages 155-165

    4. Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation

      • Jordan A. Brown, J. David Sweatt, Garrett A. Kaas
      Pages 167-188

    5. Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function

      • Cara J. Westmark, Pamela R. Westmark
      Pages 189-197

  5. Clinical Applications

    1. Front Matter

      Pages 199-199
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    2. Fractionation of Subcellular Compartments from Human Brain Tissue

      • Toni M. Mueller, Pitna Kim, James H. Meador-Woodruff
      Pages 201-223

    3. Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection

      • William J. Scotton, Abid Karim, Saiju Jacob
      Pages 225-255
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Keywords

About this book

This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications.


Editors and Affiliations

  • Department of Neurology, University of Wisconsin, Madison, USA

    Corinna Burger

  • ANSER, Homeland Security Studies and Analysis Institute, Falls Church, USA

    Margaret Jo Velardo

Bibliographic Information

  • Book Title: Glutamate Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Corinna Burger, Margaret Jo Velardo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9077-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9076-4Published: 01 February 2019

  • eBook ISBN: 978-1-4939-9077-1Published: 01 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 260

  • Number of Illustrations: 19 b/w illustrations, 48 illustrations in colour

  • Topics: Neurosciences

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