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Oligodendrocytes

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. David A. Lyons

    1. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Linde Kegel

    1. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1936)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System

    • Sueli Marques, David van Bruggen, Gonçalo Castelo-Branco
    Pages 1-21

  3. Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesions for RNA Sequencing

    • Claire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, Veronique E. Miron
    Pages 23-36

  4. Myelin: Methods for Purification and Proteome Analysis

    • Michelle S. Erwig, Dörte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer et al.
    Pages 37-63

  5. In Vitro Generation and Electrophysiological Characterization of OPCs and Oligodendrocytes from Human Pluripotent Stem Cells

    • Dario Magnani, Siddharthan Chandran, David J. A. Wyllie, Matthew R. Livesey
    Pages 65-77

  6. Isolation and Culture of Oligodendrocytes

    • Marie-Theres Weil, Giselheid Schulz-Ëberlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schäfer, Christina Müller et al.
    Pages 79-95

  7. A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation

    • Marie E. Bechler
    Pages 97-110

  8. Oligodendrocyte–Neuron Myelinating Coculture

    • Matthew Swire, Charles ffrench-Constant
    Pages 111-128

  9. Teasing of Ventral Spinal Cord White Matter Fibers for the Analysis of Central Nervous System Nodes of Ranvier

    • Andrew A. Jarjour, Diane L. Sherman
    Pages 129-139

  10. Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices

    • Sylvia Agathou, Ragnhildur Thóra Káradóttir
    Pages 141-168

  11. Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord

    • Sowmya Sekizar, Anna Williams
    Pages 169-183

  12. Forward Genetic Screen Using Zebrafish to Identify New Genes Involved in Myelination

    • Linde Kegel, Maria Rubio, Rafael G. Almeida, Silvia Benito, Anna Klingseisen, David A. Lyons
    Pages 185-209

  13. Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination

    • Jill M. Williamson, David A. Lyons, Rafael G. Almeida
    Pages 211-225

  14. A Drug-Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation

    • Marja J. Karttunen, David A. Lyons
    Pages 227-238

  15. Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis

    • Abdelkrim Mannioui, Bernard Zalc
    Pages 239-248

  16. Conditional Mutagenesis in Oligodendrocyte Lineage Cells

    • Sandra Goebbels, Klaus-Armin Nave
    Pages 249-274

  17. Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage

    • Jaime Eugenin von Bernhardi, Leda Dimou
    Pages 275-294

  18. The DTA Mouse Model for Oligodendrocyte Ablation and CNS Demyelination

    • Maria Traka
    Pages 295-310

  19. Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease

    • John N. Mariani, Lisa Zou, Steven A. Goldman
    Pages 311-331

  20. A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodendrocytes

    • Graham R. Campbell, Don J. Mahad
    Pages 333-342
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Keywords

About this book

This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function.

Editors and Affiliations

  • Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    David A. Lyons, Linde Kegel

Bibliographic Information

  • Book Title: Oligodendrocytes

  • Book Subtitle: Methods and Protocols

  • Editors: David A. Lyons, Linde Kegel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9072-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9070-2Published: 01 March 2019

  • eBook ISBN: 978-1-4939-9072-6Published: 28 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 411

  • Number of Illustrations: 10 b/w illustrations, 55 illustrations in colour

  • Topics: Cell Biology, Neurosciences

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