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Plant MicroRNAs

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Stefan de Folter

    1. Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Mexico

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1932)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants

    • Alma Armenta-Medina, C. Stewart Gillmor
    Pages 1-14

  3. The Plant microRNA Pathway: The Production and Action Stages

    • Joseph L. Pegler, Christopher P. L. Grof, Andrew L. Eamens
    Pages 15-39

  4. Plant miRNA Conservation and Evolution

    • Lydia Gramzow, Günter Theißen
    Pages 41-50

  5. Bioinformatic Analysis of Small RNA Sequencing Libraries

    • Ricardo A. Chávez Montes, Fabiola Jaimes-Miranda, Stefan de Folter
    Pages 51-63

  6. An Overview of miRNA and miRNA Target Analysis Tools

    • Anna Lukasik, Piotr Zielenkiewicz
    Pages 65-87

  7. miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences

    • Ting Zhang, Lie Ju, Jingjing Zhai, Yujia Song, Jie Song, Chuang Ma
    Pages 89-97

  8. Prediction of Plant miRNA Targets

    • Priyanka Pandey, Prashant K. Srivastava, Shree P. Pandey
    Pages 99-107

  9. Isolation and Detection Methods of Plant miRNAs

    • Pedro Fernando Vera-Hernández, Stefan de Folter, Flor de Fátima Rosas-Cárdenas
    Pages 109-120

  10. Northern Blot Analysis of microRNAs and Other Small RNAs in Plants

    • Carlos De la Rosa, José Luis Reyes
    Pages 121-129

  11. miRNA Detection by Stem-Loop RT-qPCR in Studying microRNA Biogenesis and microRNA Responsiveness to Abiotic Stresses

    • Aleksandra Smoczynska, Pawel Sega, Agata Stepien, Katarzyna Knop, Artur Jarmolowski, Andrzej Pacak et al.
    Pages 131-150

  12. Detection of miRNAs by Tissue Printing and Dot Blot Hybridization

    • Marcelino Martínez Núñez, Stefan de Folter, Flor de Fátima Rosas-Cárdenas
    Pages 151-157

  13. In Situ Localization of Small RNAs in Plants

    • Cristina F. Marco, Damianos S. Skopelitis, Marja C. P. Timmermans
    Pages 159-173

  14. Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs

    • Andrea Tovar-Aguilar, Karla A. Sánchez-Elizondo, Alejandra Rodríguez-Rodríguez, Marcos I. González-Jaime, Genaro Patiño-López, Vadim Perez-Koldenkova et al.
    Pages 175-185

  15. A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy

    • Andrea Gómez-Felipe, Stefan de Folter
    Pages 187-195

  16. Degradome Sequencing in Plants

    • Shih-Shun Lin, Yihua Chen, Mei-Yeh Jade Lu
    Pages 197-213

  17. Functional Analysis of Root microRNAs by a Constitutive Overexpression Approach in a Composite Plant System

    • Damien Formey, José Ángel Martín-Rodríguez, Georgina Hernández
    Pages 215-226

  18. The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis

    • Gigi Wong, Anthony A. Millar
    Pages 227-238

  19. Mimicry Technology: A Versatile Tool for Small RNA Suppression

    • Luis Manuel Villar-Martin, Ignacio Rubio-Somoza
    Pages 239-245

  20. Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants

    • Alberto Carbonell
    Pages 247-260
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Keywords

About this book

This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.

Editors and Affiliations

  • Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, Mexico

    Stefan de Folter

Bibliographic Information

  • Book Title: Plant MicroRNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Stefan de Folter

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9042-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9041-2Published: 31 January 2019

  • eBook ISBN: 978-1-4939-9042-9Published: 30 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 363

  • Number of Illustrations: 22 b/w illustrations, 48 illustrations in colour

  • Topics: Plant Genetics and Genomics, Gene Expression

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