Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1930)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
About this book
This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields ofimmunology, T-cell biology, biochemistry, molecular biology, and imaging.
Editors and Affiliations
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Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
Navin Kumar Verma
Bibliographic Information
Book Title: T-Cell Motility
Book Subtitle: Methods and Protocols
Editors: Navin Kumar Verma
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9036-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9035-1Published: 05 January 2019
eBook ISBN: 978-1-4939-9036-8Published: 04 January 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 158
Number of Illustrations: 3 b/w illustrations, 28 illustrations in colour
Topics: Immunology, Cell Biology