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Cancer Metabolism

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Majda Haznadar

    1. National Cancer Institute, North Bethesda, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essentail for reproducible results
  • Contains key note and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1928)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction

    • Daniel R. Crooks, Teresa W.-M. Fan, W. Marston Linehan
    Pages 1-27

  3. Imaging Cancer Metabolism with Positron Emission Tomography (PET)

    • Timothy H. Witney, David Y. Lewis
    Pages 29-44

  4. Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells

    • Debanti Sengupta, Guillem Pratx
    Pages 45-53

  5. Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation Dynamics in Cancer Cell Metabolism and Epigenetics

    • Alice C. Newman, Christiaan F. Labuschagne, Karen H. Vousden, Oliver D. K. Maddocks
    Pages 55-67

  6. Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM)

    • Sarah Creed, Matthew McKenzie
    Pages 69-76

  7. Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells

    • David Kung-Chun Chiu, Misty Shuo Zhang, Aki Pui-Wah Tse, Carmen Chak-Lui Wong
    Pages 77-99

  8. Determination of Polarization of Resident Macrophages and Their Effect on the Tumor Microenvironment

    • Ioannis S. Pateras, Tomer Cooks
    Pages 101-112

  9. Quantitation of Macropinocytosis in Cancer Cells

    • Koen M. O. Galenkamp, Basheer Alas, Cosimo Commisso
    Pages 113-123

  10. Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation

    • Simone Sidoli, Sophie Trefely, Benjamin A. Garcia, Alessandro Carrer
    Pages 125-147

  11. Methods to Measure Autophagy in Cancer Metabolism

    • Natalia von Muhlinen
    Pages 149-173

  12. Lipidomic Analysis of Cancer Cell and Tumor Tissues

    • Islam Sk Ramiz, Soumen Kanti Manna
    Pages 175-204

  13. Mass Spectrometry-Based Profiling of Metabolites in Human Biofluids

    • Tanushree Chakraborty, Soumen Kanti Manna
    Pages 205-234

  14. Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile Through Extraction and Derivatization of Polar Metabolites

    • Jodi Rattner, Farshad Farshidfar, Oliver F. Bathe
    Pages 235-249

  15. Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy

    • Beata Mickiewicz, M. Eric Hyndman, Hans J. Vogel
    Pages 251-274

  16. Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging

    • Shibdas Banerjee, Soumen Kanti Manna
    Pages 275-297

  17. Compositional Analysis of the Human Microbiome in Cancer Research

    • Elisa Morales, Jun Chen, K. Leigh Greathouse
    Pages 299-335

  18. Assessing Metabolic Dysregulation in Muscle During Cachexia

    • Myriam Y. Hsu, Paolo E. Porporato, Elisabeth Wyart
    Pages 337-352

  19. Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells

    • Jing Zhang, Qing Zhang
    Pages 353-363

  20. Metabolic Profiling of Live Cancer Tissues Using NAD(P)H Fluorescence Lifetime Imaging

    • Thomas S. Blacker, Michael D. E. Sewell, Gyorgy Szabadkai, Michael R. Duchen
    Pages 365-387
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Keywords

About this book

This volume details common experimental approaches in studies designed to illuminate various processes used to study cancer metabolism.  Chapters are divided into three parts, the first part focuses on protocols commonly utilized in cancer metabolism studies, such as protocols comprising stable isotope labeling methods, protocols for studying glycolysis, gluconeogenesis and mitochondrial metabolism.  The second part of the book describes methods used for generating hypotheses and identifying cancer markers, such as mass spectrometry- and NMR-based profiling tools. Last but not least, the third part is an overview of vital and actively researched topics in the field of cancer metabolism, as well as computational methodological approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cancer Metabolism: Methods and Protocols aims to be an essential compilation of protocols utilized in many laboratories.

Editors and Affiliations

  • National Cancer Institute, North Bethesda, USA

    Majda Haznadar

Bibliographic Information

  • Book Title: Cancer Metabolism

  • Book Subtitle: Methods and Protocols

  • Editors: Majda Haznadar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9027-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9026-9Published: 07 February 2019

  • eBook ISBN: 978-1-4939-9027-6Published: 06 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 495

  • Number of Illustrations: 19 b/w illustrations, 60 illustrations in colour

  • Topics: Cancer Research

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