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  • © 2019

Recombinant Protein Production in Yeast

  • Include cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1923)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
  2. Established and Upcoming Yeast Expression Systems

    • Burcu Gündüz Ergün, Damla HüccetoÄŸulları, Sibel Öztürk, Eda Çelik, Pınar Çalık
    Pages 1-74
  3. Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts

    • Richard J. Zahrl, Brigitte Gasser, Diethard Mattanovich, Pau Ferrer
    Pages 75-95
  4. Pichia pastoris Promoters

    • Aysun TürkanoÄŸlu Özçelik, Semiramis Yılmaz, Mehmet Inan
    Pages 97-112
  5. Selection of the Optimal Yeast Host for the Synthesis of Recombinant Enzymes

    • Felix Bischoff, Martin Giersberg, Falko Matthes, Tobias Schwalenberg, Sebastian Worch, Gotthard Kunze
    Pages 113-132
  6. Selection of Heterologous Protein-Producing Strains in Yarrowia lipolytica

    • Paul Soudier, Macarena Larroude, Ewelina CeliÅ„ska, Tristan Rossignol, Jean-Marc Nicaud
    Pages 153-168
  7. Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics

    • Roslyn M. Bill, Annegret Ulke-Lemée, Stephanie P. Cartwright, Rena Far, Jay Kim, Justin A. MacDonald
    Pages 187-209
  8. CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris

    • Thomas Gassler, Lina Heistinger, Diethard Mattanovich, Brigitte Gasser, Roland Prielhofer
    Pages 211-225
  9. Tailoring N-Glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae

    • Mari A. Piirainen, Heidi Salminen, Alexander D. Frey
    Pages 227-241
  10. Improving the Production of Cofactor-Containing Proteins: Production of Human Hemoglobin in Yeast

    • Olena P. Ishchuk, José L. Martínez, Dina Petranovic
    Pages 243-264
  11. Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production

    • Anita Emmerstorfer-Augustin, Tamara Wriessnegger, Melanie Hirz, Guenther Zellnig, Harald Pichler
    Pages 265-285
  12. Yeast Surface Display and Cell Sorting of Antigen-Binding Fc Fragments

    • Flávio Sádio, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Rüker, Gordana Wozniak-Knopp
    Pages 287-308
  13. Efficient Development of a Mixed Feed Process for Pichia pastoris

    • David Johannes Wurm, Oliver Spadiut
    Pages 323-333
  14. LC-MS Analysis of (Glyco-)Proteins of Pichia pastoris

    • Clemens Grünwald-Gruber, Friedrich Altmann
    Pages 351-360
  15. Assessing the Quality of Recombinant Products Made in Yeast

    • Karola Vorauer-Uhl, Gabriele Lhota
    Pages 361-384

About this book

This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications. 


Editors and Affiliations

  • Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU) and Austrian Centre of Industrial Biotechnology (acib), Vienna, Austria

    Brigitte Gasser, Diethard Mattanovich

About the editors

  

Bibliographic Information

  • Book Title: Recombinant Protein Production in Yeast

  • Editors: Brigitte Gasser, Diethard Mattanovich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9024-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9023-8Published: 09 February 2019

  • eBook ISBN: 978-1-4939-9024-5Published: 08 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 431

  • Number of Illustrations: 14 b/w illustrations, 46 illustrations in colour

  • Topics: Biotechnology, Protein Science

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access