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Odontogenesis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Petros Papagerakis

    1. School of Dentistry, University of Michigan, Ann Arbor, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1922)

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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Protocols for Establishment of Dental Cells Lines and Animal Models

    1. Front Matter

      Pages 1-1
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    2. Microdissection and Isolation of Mouse Dental Epithelial Cells of Continuously Growing Mouse Incisors

      • Hidemitsu Harada, Keishi Otsu
      Pages 3-11

    3. Establishment of an Immortalized Mouse Bmp2 Knockout Dental Papilla Mesenchymal Cell Line

      • Wen’an Xu, Shuo Chen
      Pages 13-19

    4. Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells

      • Elizabeth Guirado, Youbin Zhang, Anne George
      Pages 21-27

    5. Isolation of Dental Stem Cell-Enriched Populations from Continuously Growing Mouse Incisors

      • Anamaria Balic
      Pages 29-37

    6. Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentinogenesis

      • Yan Jing, Chaoyuan Li, Jian Q. Feng
      Pages 39-48

    7. Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-Mesenchymal Interactions

      • Lucia Jimenez-Rojo, Thimios A. Mitsiadis
      Pages 49-55

  3. Protocols for Dental Stem Cells and Tissue Regeneration

    1. Front Matter

      Pages 57-57
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    2. Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, and Primary Teeth Stem Cells—Isolation, Characterization, and Expansion for Tissue Engineering

      • Mey Al-Habib, George T. -J. Huang
      Pages 59-76

    3. Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal Cells Subpopulations with Multiparametric Flow Cytometry

      • Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup, Emeline Perrier-Groult, Andreas Mueller, Frédéric Mallein-Gerin et al.
      Pages 77-90

    4. Dental Pulp Stem Cells: Isolation, Characterization, Expansion, and Odontoblast Differentiation for Tissue Engineering

      • Qing Dong, Yuanyuan Wang, Fatemeh Mohabatpour, Li Zheng, Silvana Papagerakis, Daniel Chen et al.
      Pages 91-101

    5. In Vitro Analysis of Intramolecular Signaling Events in PDLSCs Using Confocal and TIRF Microscopy

      • Annette Merkel, Anne George
      Pages 103-110

    6. A Mouse Model to Study Reparative Dentinogenesis

      • R. C. Babb, D. Chandrasekaran, L. K. Zaugg, P. T. Sharpe
      Pages 111-119

    7. Multiwalled Carbon Nanotubes for Dental Applications

      • Petros Kechagioglou, Eleftherios Andriotis, Petros Papagerakis, Silvana Papagerakis
      Pages 121-128

    8. Peptide-Mediated Biomimetic Regrowth of Human Enamel In Situ

      • Kaushik Mukherjee, Qichao Ruan, Janet Moradian-Oldak
      Pages 129-138

    9. Bioengineering Tooth Bud Constructs Using GelMA Hydrogel

      • Elizabeth E. Smith, Pamela C. Yelick
      Pages 139-150

    10. Whole-Mount In Situ Hybridization of Mouse Embryos Using DIG-Labeled RNA Probes

      • Jingyi Wu, Xiaofang Wang
      Pages 151-159

  4. Protocols for Studying Gene and Protein Expression

    1. Front Matter

      Pages 161-161
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    2. In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes

      • Jingyi Wu, Jian Q. Feng, Xiaofang Wang
      Pages 163-171
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Keywords

About this book

This volume provides methods and approaches to study genetic and environmental regulatory controls on odontogenesis. Chapters guide readers through protocols for isolation and characterization of both epithelial and mesenchymal dental cells, methods on isolation, phenotypic characterization, expansion, differentiation, immunofluorescence, in situ hybridization, immunohistochemistry, imaging protocols, rodent dental fluorosis model, 3D assessment of crown size, dental diseases models, next generation sequencing, genetic and epigenetic studies, genome-wide association studies as well as clinical protocols for measurement of early childhood caries and saliva,  and supragingival fluids and biofilm collection and subsequent analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Odontogenesis: Methods and Protocols aims to guide researchers towards elucidating the secrets and mysteries of a fascinating and unique organ, the tooth.

Editors and Affiliations

  • School of Dentistry, University of Michigan, Ann Arbor, USA

    Petros Papagerakis

Bibliographic Information

  • Book Title: Odontogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Petros Papagerakis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9012-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9011-5Published: 06 March 2019

  • eBook ISBN: 978-1-4939-9012-2Published: 05 March 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 565

  • Number of Illustrations: 17 b/w illustrations, 135 illustrations in colour

  • Topics: Molecular Medicine, Dentistry

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