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  • © 2019

Vertebrate Embryogenesis

Embryological, Cellular, and Genetic Methods

  • Includes cutting-edge, fully updated techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1920)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
  2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos

    • Thao Nguyen, Timothy J. Mitchison, Martin WĂĽhr
    Pages 17-32
  3. Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

    • Alexander A. Tokmakov, Ken-Ichi Sato
    Pages 41-57
  4. Embryological and Genetic Manipulation of Chick Development

    • Laura S. Gammill, Bridget Jacques-Fricke, Julaine Roffers-Agarwal
    Pages 75-97
  5. Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos

    • Ana Paula Azambuja, Marcos Simoes-Costa
    Pages 99-110
  6. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq)

    • Karoline Holler, Jan Philipp Junker
    Pages 129-141
  7. Ex Utero Culture and Imaging of Mouse Embryos

    • Sonja Nowotschin, Vidur Garg, Anna Piliszek, Anna-Katerina Hadjantonakis
    Pages 163-182
  8. Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

    • Edwina McGlinn, Miriam A. Holzman, Jennifer H. Mansfield
    Pages 183-218
  9. Reptile Embryology and Regeneration

    • Matthew K. Vickaryous, Emily A. B. Gilbert
    Pages 219-246
  10. Manipulation of Developmental Function in Turtles with Notes on Alligators

    • Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, Judith A. Cebra-Thomas
    Pages 247-263
  11. Methods for Isolating the Balbiani Body/Germplasm from Xenopus laevis Oocytes

    • Amanda Butler, Dawn Owens, Mary Lou King, Tristan Aguero
    Pages 265-275
  12. Visualizing the Balbiani Body in Zebrafish Oocytes

    • KathyAnn L. Lee, Florence L. Marlow
    Pages 277-293
  13. Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis

    • Allison Jamieson-Lucy, Mary C. Mullins
    Pages 295-302
  14. Generation of Xenopus Haploid, Triploid, and Hybrid Embryos

    • Romain Gibeaux, Rebecca Heald
    Pages 303-315
  15. Germ Cell Transplantation in Avian Species

    • Young Hyun Park, Young Min Kim, Jae Yong Han
    Pages 317-326

About this book

This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.  

Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Laboratory of Genetics, University of Wisconsin–Madison, Madison, USA

    Francisco J. Pelegri

Bibliographic Information

  • Book Title: Vertebrate Embryogenesis

  • Book Subtitle: Embryological, Cellular, and Genetic Methods

  • Editors: Francisco J. Pelegri

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9009-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9008-5Published: 09 February 2019

  • eBook ISBN: 978-1-4939-9009-2Published: 08 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 434

  • Number of Illustrations: 12 b/w illustrations, 70 illustrations in colour

  • Topics: Embryology, Animal Models, Animal Genetics and Genomics

Buy it now

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access