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Vertebrate Embryogenesis

Embryological, Cellular, and Genetic Methods

  • Book
  • © 2019

Overview

Editors:
  1. Francisco J. Pelegri

    1. Laboratory of Genetics, University of Wisconsin–Madison, Madison, USA

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  • Includes cutting-edge, fully updated techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1920)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments

    • Douglas W. Houston
    Pages 1-16

  3. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos

    • Thao Nguyen, Timothy J. Mitchison, Martin Wühr
    Pages 17-32

  4. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos

    • Michael D. Sheets
    Pages 33-39

  5. Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

    • Alexander A. Tokmakov, Ken-Ichi Sato
    Pages 41-57

  6. Membrane Microdomains as Platform to Study Membrane-Associated Events During Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis

    • Ken-Ichi Sato, Alexander A. Tokmakov
    Pages 59-73

  7. Embryological and Genetic Manipulation of Chick Development

    • Laura S. Gammill, Bridget Jacques-Fricke, Julaine Roffers-Agarwal
    Pages 75-97

  8. Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos

    • Ana Paula Azambuja, Marcos Simoes-Costa
    Pages 99-110

  9. Experimental Manipulation of Ploidy in Zebrafish Embryos and Its Application in Genetic Screens

    • Triveni Menon, Sreelaja Nair
    Pages 111-128

  10. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq)

    • Karoline Holler, Jan Philipp Junker
    Pages 129-141

  11. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos

    • Clare E. Buckley
    Pages 143-162Open Access

  12. Ex Utero Culture and Imaging of Mouse Embryos

    • Sonja Nowotschin, Vidur Garg, Anna Piliszek, Anna-Katerina Hadjantonakis
    Pages 163-182

  13. Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

    • Edwina McGlinn, Miriam A. Holzman, Jennifer H. Mansfield
    Pages 183-218

  14. Reptile Embryology and Regeneration

    • Matthew K. Vickaryous, Emily A. B. Gilbert
    Pages 219-246

  15. Manipulation of Developmental Function in Turtles with Notes on Alligators

    • Jacqueline E. Moustakas-Verho, Rebecca McLennan, Jennifer Spengler, Paul M. Kulesa, Judith A. Cebra-Thomas
    Pages 247-263

  16. Methods for Isolating the Balbiani Body/Germplasm from Xenopus laevis Oocytes

    • Amanda Butler, Dawn Owens, Mary Lou King, Tristan Aguero
    Pages 265-275

  17. Visualizing the Balbiani Body in Zebrafish Oocytes

    • KathyAnn L. Lee, Florence L. Marlow
    Pages 277-293

  18. Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis

    • Allison Jamieson-Lucy, Mary C. Mullins
    Pages 295-302

  19. Generation of Xenopus Haploid, Triploid, and Hybrid Embryos

    • Romain Gibeaux, Rebecca Heald
    Pages 303-315

  20. Germ Cell Transplantation in Avian Species

    • Young Hyun Park, Young Min Kim, Jae Yong Han
    Pages 317-326
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Keywords

About this book

This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.  

Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Laboratory of Genetics, University of Wisconsin–Madison, Madison, USA

    Francisco J. Pelegri

Bibliographic Information

  • Book Title: Vertebrate Embryogenesis

  • Book Subtitle: Embryological, Cellular, and Genetic Methods

  • Editors: Francisco J. Pelegri

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9009-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9008-5Published: 09 February 2019

  • eBook ISBN: 978-1-4939-9009-2Published: 08 February 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 434

  • Number of Illustrations: 12 b/w illustrations, 70 illustrations in colour

  • Topics: Embryology, Animal Models, Animal Genetics and Genomics

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