Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1915)
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Table of contents (20 protocols)
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Production and Purification of Calpains
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Determination of Calpain Localization, Expression, and Activity
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Identification of Calpain Substrates and Calpain-Activated Protein Functions
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Interrogation of Calpastatin, The Endogenous Calpain Inhibitor
Keywords
About this book
This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Calpain: Methods and Protocols is a valuable resource for researchers and scientists who want to learn moreabout this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases.
Editors and Affiliations
Bibliographic Information
Book Title: Calpain
Book Subtitle: Methods and Protocols
Editors: Jeannette S. Messer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8988-1
Publisher: Springer New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-8987-4Published: 08 January 2019
eBook ISBN: 978-1-4939-8988-1Published: 08 January 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 276
Number of Illustrations: 29 b/w illustrations, 37 illustrations in colour
Topics: Protein Science