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  • © 2019

Co-Immunoprecipitation Methods for Brain Tissue

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 144)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xiii
  2. Co-immunoprecipitation as a Useful Tool for Detection of G Protein-Coupled Receptor Oligomers

    • Kirill Shumilov, Alejandra Valderrama-Carvajal, María García-Bonilla, Alicia Rivera
    Pages 109-122
  3. Co-immunoprecipitation of Membrane-Bound Receptors from Subsynaptic Compartments

    • Wilber Romero-Fernandez, Maria Garcia-Barcelo, Yunis Perez-Betancourt
    Pages 137-145
  4. Co-immunoprecipitation (Co-IP) of G Protein-Coupled Receptor (GPCR)-Receptor Tyrosine Kinase (RTK) Complexes from the Dorsal Hippocampus of the Rat Brain

    • Michael Di Palma, Manuel Narvaez, Mariana Pita-Rodríguez, Chiara Contri, Martina Zannoni, Riccardo Cuppini et al.
    Pages 157-164
  5. Back Matter

    Pages 165-166

About this book

This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Co-Immunoprecipitation Methods for Brain Tissue is a valuable resource for any researcher interesting in learning more about this developing field.



Editors and Affiliations

  • Department of Psychiatry, Saitama Medical University, Saitama, Japan

    Yuji Odagaki

  • Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Dasiel O. Borroto-Escuela

Bibliographic Information

  • Book Title: Co-Immunoprecipitation Methods for Brain Tissue

  • Editors: Yuji Odagaki, Dasiel O. Borroto-Escuela

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-8985-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8984-3Published: 14 December 2018

  • eBook ISBN: 978-1-4939-8985-0Published: 13 December 2018

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XIII, 166

  • Number of Illustrations: 9 b/w illustrations, 19 illustrations in colour

  • Topics: Neurosciences, Protein Science

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access