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Cell-free DNA as Diagnostic Markers

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Valentina Casadio

    1. Biosciences Laboratory, IRCCS I.R.S.T, Meldola, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Samanta Salvi

    1. Biosciences Laboratory, IRCCS I.R.S.T, Meldola, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1909)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
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  2. An Overview

    1. Front Matter

      Pages 1-1
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    2. Cell-Free DNA: Applications in Different Diseases

      • Rossella Ranucci
      Pages 3-12

    3. Cell-Free DNA: An Overview of Sample Types and Isolation Procedures

      • Filippo Martignano
      Pages 13-27

  4. Methods in Cancer

    1. Front Matter

      Pages 75-75
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    2. Cell-Free DNA Integrity: Applications

      • Sara Ravaioli
      Pages 77-83

    3. Copy Number Variation Analysis on Cell-Free Serum DNA

      • Heidi Schwarzenbach
      Pages 85-93

    4. Studying Copy Number Variations in Cell-Free DNA: The Example of AR in Prostate Cancer

      • Samanta Salvi, Valentina Casadio
      Pages 95-103

    5. dPCR Mutational Analyses in Cell-Free DNA: A Comparison with Tissues

      • Takashi Takeshita, Hirotaka Iwase
      Pages 105-118

    6. From cfDNA to Sequencing: Workflows and Potentials

      • Michela Tebaldi, Samanta Salvi
      Pages 119-125

  5. Epigenetic Modifications

    1. Front Matter

      Pages 127-127
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    2. Epigenetic Characterization of Cell-Free DNA

      • Giorgia Gurioli
      Pages 129-135

    3. Quantitative Methylation-Specific PCR: A Simple Method for Studying Epigenetic Modifications of Cell-Free DNA

      • Luca Sigalotti, Alessia Covre, Francesca Colizzi, Elisabetta Fratta
      Pages 137-162

  6. Fetal and Pediatric Diseases

    1. Front Matter

      Pages 163-163
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    2. Cell-Free DNA in Pediatric Rhabdomyosarcoma: Potential and Challenges

      • Lucia Tombolan, Angelica Zin, Gianni Bisogno
      Pages 165-175

    3. Bioinformatics Pipeline for Accurate Quantification of Fetal DNA Fraction in Maternal Plasma

      • Meng Ni, Xianlu Laura Peng, Peiyong Jiang
      Pages 177-180

  7. Physical Activity

    1. Front Matter

      Pages 181-181
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Keywords

About this book

This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cell-Free DNA as Diagnostic Markers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Biosciences Laboratory, IRCCS I.R.S.T, Meldola, Italy

    Valentina Casadio, Samanta Salvi

Bibliographic Information

  • Book Title: Cell-free DNA as Diagnostic Markers

  • Book Subtitle: Methods and Protocols

  • Editors: Valentina Casadio, Samanta Salvi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8973-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8972-0Published: 23 December 2018

  • eBook ISBN: 978-1-4939-8973-7Published: 22 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 224

  • Number of Illustrations: 2 b/w illustrations, 27 illustrations in colour

  • Topics: Human Genetics, Cancer Research

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