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Brain-Derived Neurotrophic Factor (BDNF)

  • Book
  • © 2019

Overview

Editors:
  1. Carlos B. Duarte

    1. Center for Neuroscience and Cell Biology and Department of Life Sciences, University of Coimbra, Coimbra, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  2. Enrico Tongiorgi

    1. BRAIN Center for Neuroscience, Department of Life Sciences, University of Trieste, Trieste, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 143)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
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  3. Transcripts of BDNF

    1. Front Matter

      Pages 11-11
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    2. Usage of Bacterial Artificial Chromosomes for Studying BDNF Gene Regulation in Primary Cultures of Cortical Neurons and Astrocytes

      • Kaur Jaanson, Angela Pärn, Tõnis Timmusk
      Pages 13-25

    3. Detecting Single and Multiple BDNF Transcripts by In Situ Hybridization in Neuronal Cultures and Brain Sections

      • Andrea Colliva, Kristen R. Maynard, Keri Martinowich, Enrico Tongiorgi
      Pages 27-53

    4. Studying BDNF/TrkB Signaling: Transcriptome Analysis from a Limited Number of Purified Adult or Aged Murine Brain Neurons

      • Chinnavuth Vatanashevanopakorn, Amit Grover, Arup R. Nath, Kevin Clark, Paul Sopp, Claus Nerlov et al.
      Pages 55-76

    5. Studying BDNF/TrkB Signaling: High-Throughput Microfluidic Gene Expression Analysis from Rare or Limited Samples of Adult and Aged Central Neurons

      • Arup R. Nath, Roy Drissen, Fei Guo, Claus Nerlov, Liliana Minichiello
      Pages 77-86

  4. Protein Forms of BDNF

    1. Front Matter

      Pages 87-87
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    2. Detecting BDNF Protein Forms by ELISA, Western Blot, and Immunofluorescence

      • Stefano Donegà, Enrico Tongiorgi
      Pages 89-103

    3. Methodology for Detecting and Tracking Brain-Derived Neurotrophic Factor Complexes in Neurons Using Single Quantum Dots

      • Anke Vermehren-Schmaedick, Thomas Jacob, Tania Q. Vu
      Pages 105-118

    4. Recording Activity-Dependent Release of BDNF from Hippocampal Neurons

      • Tanja Brigadski, Petra Lichtenecker, Volkmar Lessmann
      Pages 119-129

  5. Receptors of BDNF

    1. Front Matter

      Pages 131-131
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    2. Ultrastructural Localization of BDNF and trkB Receptors

      • Chiara Salio, Adalberto Merighi
      Pages 133-148

    3. Analysis of TrkB Receptor Activity Using FRET Sensors

      • Charles E. Hall, James O. McNamara, Ryohei Yasuda
      Pages 149-157

  6. Signaling Cascades of BDNF

    1. Front Matter

      Pages 159-159
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    2. BDNF-Induced Intracellular Signaling

      • João R. Gomes, Andrea Lobo, Carlos B. Duarte, Mário Grãos
      Pages 161-183

    3. A Microfluidic Culture Platform for Neurotrophin Signaling Studies

      • Rui O. Costa, Tânia Perestrelo, Diogo Tomé, Ramiro D. Almeida
      Pages 185-201

  7. BDNF-Induced Protein Synthesis and Synaptic Regulation

    1. Front Matter

      Pages 203-203
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    2. BDNF-Induced Local Protein Synthesis in Synaptoneurosomes Assessed with Click-iT L-Azidohomoalanine

      • Victor Briz, Michel Baudry
      Pages 205-215
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Keywords

About this book

This volume discusses the latest techniques and methodologies used in the neurotrophin field to study the physiology of the Brain-Derived Neurotrophic Factor (BDNF). The book provides the tools essential to any researcher that intends to explore the peculiar regulation of this neurotrophin from gene expression to its release and the signaling by TrkB receptors, as well as to study the neuronal responses to BDNF or the role of neurotrophin in various neurological diseases. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting edge and comprehensive, Brain-Derived Neurotrophic Factor(BDNF) is a valuable resource for researchers who want to further study the diversity of physiological roles of this important growth factor.


Editors and Affiliations

  • Center for Neuroscience and Cell Biology and Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    Carlos B. Duarte

  • BRAIN Center for Neuroscience, Department of Life Sciences, University of Trieste, Trieste, Italy

    Enrico Tongiorgi

Bibliographic Information

  • Book Title: Brain-Derived Neurotrophic Factor (BDNF)

  • Editors: Carlos B. Duarte, Enrico Tongiorgi

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-8970-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8969-0Published: 10 July 2019

  • eBook ISBN: 978-1-4939-8970-6Published: 09 July 2019

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 258

  • Number of Illustrations: 4 b/w illustrations, 31 illustrations in colour

  • Topics: Neurosciences

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