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Microfluidic Electrophoresis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Debashis Dutta

    1. Department of Chemistry, University of Wyoming, Laramie, USA

    View editor publications

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1906)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Fabrication of Glass Microfluidic Devices

    • Christopher T. Culbertson, Jay Sibbitts, Kathleen Sellens, Shu Jia
    Pages 1-12

  3. Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices

    • Ashis Kumar Sen, Abhishek Raj, Utsab Banerjee, Sk Rameez Iqbal
    Pages 13-54

  4. Sample Injection Techniques

    • James M. Karlinsey
    Pages 55-64

  5. Sample Preconcentration Protocols in Microfluidic Electrophoresis

    • Fumihiko Kitagawa, Koji Otsuka
    Pages 65-78

  6. Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection

    • Lucas Paines Bressan, Dosil Pereira de Jesus, Dulan Bandara Gunasekara, Susan Marie Lunte, José Alberto Fracassi da Silva
    Pages 79-85

  7. Micellar Electrokinetic Chromatography

    • Braden C. Giordano, Ronald Siefert, Greg E. Collins
    Pages 87-97

  8. Microchip Isotachophoresis: Analysis of Pharmaceuticals

    • Marián Masár, Jasna Hradski
    Pages 99-111

  9. Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination

    • Stefan Nagl
    Pages 113-124

  10. Nanochannel Gradient Separations

    • Michael A. Startsev, David W. Inglis
    Pages 125-132

  11. Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications

    • Cyro L. S. Chagas, Thiago M. G. Cardoso, Wendell K. T. Coltro
    Pages 133-142

  12. Band Broadening Theories in Capillary Electrophoresis

    • Sandip Ghosal
    Pages 143-166

  13. Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation

    • Debashis Dutta
    Pages 167-195

  14. Microchip Electrophoresis Tools for the Analysis of Small Molecules

    • Federico J. V. Gomez, María Fernanda Silva
    Pages 197-206

  15. Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS)—Swab Sample-In to DNA Profile-Out

    • Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, Frederic Zenhausern
    Pages 207-224

  16. Achieving Stable Electrospray Ionization Mass Spectrometry Detection from Microfluidic Chips

    • Iulia M. Lazar
    Pages 225-237

  17. Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow

    • Ling Xia, Debashis Dutta
    Pages 239-249

  18. Back Matter

    Pages 251-255
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Keywords

About this book

This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.

Editors and Affiliations

  • Department of Chemistry, University of Wyoming, Laramie, USA

    Debashis Dutta

Bibliographic Information

  • Book Title: Microfluidic Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Debashis Dutta

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8964-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8963-8Published: 29 November 2018

  • eBook ISBN: 978-1-4939-8964-5Published: 28 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 255

  • Number of Illustrations: 46 b/w illustrations, 47 illustrations in colour

  • Topics: Electrophoresis, Biotechnology

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