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Autoantibodies

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Gunnar Houen

    1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1901)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Autoantibodies as Diagnostic Tools

    • Gunnar Houen
    Pages 1-11

  3. Nonspecific Binding in Immunoassays for Autoantibodies

    • Gunnar Houen
    Pages 13-17

  4. Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on Hep-2 Cells

    • Alessandra Dellavance, Luis Eduardo Coelho Andrade
    Pages 19-46

  5. Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence

    • J. J. B. C. van Beers, J. Vanderlocht, C. Roozendaal, J. Damoiseaux
    Pages 47-62

  6. Determination of Subset-Restricted Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry

    • Firoozeh Amirbeagi, Amanda Welin, Pontus Thulin, Johan Bylund
    Pages 63-77

  7. Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry

    • Jehan Alam, Sumin Jeon, Youngnim Choi
    Pages 79-87

  8. Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry

    • Satoshi Yamashita, Nozomu Tawara
    Pages 89-94

  9. Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay

    • Hanna Davideit, Annekathrin Haberland, Sabine Bartel, Sarah Schulze-Rothe, Johannes Müller, Katrin Wenzel
    Pages 95-102

  10. Determination of Autoantibodies to Salivary Gland Antigens

    • Li Cui, Xinyuan Zhao, Shen Hu
    Pages 103-112

  11. Measurement of Autoantibodies to Gastric H+,K+-ATPase (ATP4A/B) Using a Luciferase Immunoprecipitation System (LIPS)

    • Edith Lahner, Ilaria Marzinotto, Cristina Brigatti, Howard Davidson, Janet Wenzlau, Lorenzo Piemonti et al.
    Pages 113-131

  12. Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay

    • Jacqueline Keyhani, Ezzatollah Keyhani
    Pages 133-152

  13. Antigen-Specific Detection of Autoantibodies Against Myeloperoxidase (MPO) and Proteinase 3 (PR3)

    • J. Vanderlocht, J. J. B. C. van Beers, P. C. Limburg, J. Damoiseaux, C. Roozendaal
    Pages 153-176

  14. Analysis of C3 Nephritic Factors by ELISA

    • Lillemor Skattum
    Pages 177-182

  15. Analysis of Anti-C1q Autoantibodies by Western Blot

    • Anci Verlemyr, Lennart Truedsson, Lillemor Skattum
    Pages 183-189

  16. Anti-factor H Autoantibodies Assay by ELISA

    • Marie Sénant, Marie-Agnes Dragon-Durey
    Pages 191-196

  17. Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay

    • Zhiyuan Zhao, Yong Gu, Dongmei Miao, Eric Hoffmeyer, Yu Liu, Liping Yu
    Pages 197-203

  18. Determination of CRP Autoantibodies by SPR Immunoassay

    • Qiu-Yu Li, Hai-Yun Li
    Pages 205-219

  19. Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis

    • Feliciana Real-Fernández, Federico Pratesi, Paola Migliorini, Paolo Rovero
    Pages 221-228

  20. Detection of SSA and SSB Antibodies Associated with Primary Sjögren’s Syndrome Using Enzyme-Linked Immunosorbent Assay

    • Nicole Hartwig Trier
    Pages 229-237
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Keywords

About this book

This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients.


Editors and Affiliations

  • Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

Bibliographic Information

  • Book Title: Autoantibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Gunnar Houen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8949-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8948-5Published: 12 December 2018

  • eBook ISBN: 978-1-4939-8949-2Published: 12 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 289

  • Number of Illustrations: 26 b/w illustrations, 32 illustrations in colour

  • Topics: Antibodies, Laboratory Medicine

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