Skip to main content
SpringerLink
Log in

Barley

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Wendy A. Harwood

    1. Crop Transformation Group, Department of Crop Genetics, John Innes Centre, Norwich, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and expert implementation advice

Part of the book series: Methods in Molecular Biology (MIMB, volume 1900)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. An Introduction to Barley: The Crop and the Model

    • Wendy A. Harwood
    Pages 1-5

  3. Mutation Breeding in Barley: Historical Overview

    • Ludmila Ohnoutkova
    Pages 7-19

  4. A Practical Guide to Barley Crossing

    • William T. B. Thomas, Hazel Bull, Allan Booth, Ruth Hamilton, Brian P. Forster, Jerome D. Franckowiak
    Pages 21-36

  5. Barley Anther Culture

    • Ludmila Ohnoutkova, Tomas Vlcko, Mentewab Ayalew
    Pages 37-52

  6. Isolated Microspore Culture in Barley

    • Patricio Esteves, François J. Belzile
    Pages 53-71

  7. TILLING in Barley

    • Matthias Jost, Miriam Szurman-Zubrzycka, Katarzyna Gajek, Iwona Szarejko, Nils Stein
    Pages 73-94

  8. Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings

    • Lokanadha R. Gunupuru, Alexandre Perochon, Shahin S. Ali, Steven R. Scofield, Fiona M. Doohan
    Pages 95-114

  9. Agrobacterium-Mediated Transformation of Barley Immature Embryos

    • Alison Hinchliffe, Wendy A. Harwood
    Pages 115-126

  10. Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses During Early Development

    • Agata Daszkowska-Golec, Anna Skubacz, Marzena Kurowska, MichaÅ‚ SÅ‚ota, Dorota Swiergolik, Iwona Szarejko
    Pages 127-151

  11. Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy

    • Marek Marzec, Michael Melzer
    Pages 153-166

  12. Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy

    • Sybille Mittmann, Mikel Arrieta, Luke Ramsay, Robbie Waugh, Isabelle Colas
    Pages 167-179

  13. Microarrays for High-Throughput Gene Expression Analysis of Barley

    • Jenny A. Morris, Pete E. Hedley
    Pages 181-194

  14. Genome Engineering Using TALENs

    • Goetz Hensel, Jochen Kumlehn
    Pages 195-215

  15. Creating Targeted Gene Knockouts in Barley Using CRISPR/Cas9

    • Tom Lawrenson, Wendy A. Harwood
    Pages 217-232

  16. Genotyping-by-Sequencing on the Ion Torrent Platform in Barley

    • Amina Abed, Gaétan Légaré, Sonia Pomerleau, Jérôme St-Cyr, Brian Boyle, François J. Belzile
    Pages 233-252

  17. DNA Methylation Analysis in Barley and Other Species with Large Genomes

    • Karolina Chwialkowska, Urszula Korotko, Miroslaw Kwasniewski
    Pages 253-268

  18. High-Resolution RT-PCR Analysis of Alternative Barley Transcripts

    • Craig G. Simpson, John Fuller, Paulo Rapazote-Flores, Claus-Dieter Mayer, Cristiane P. G. Calixto, Linda Milne et al.
    Pages 269-281

  19. Exome Capture for Variant Discovery and Analysis in Barley

    • Micha Bayer, Jenny A. Morris, Clare Booth, Allan Booth, Niki Uzrek, Joanne R. Russell et al.
    Pages 283-310

  20. Back Matter

    Pages 311-313
    Download chapter PDF
Back to top

Keywords

About this book

This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.

Editors and Affiliations

  • Crop Transformation Group, Department of Crop Genetics, John Innes Centre, Norwich, UK

    Wendy A. Harwood

Bibliographic Information

  • Book Title: Barley

  • Book Subtitle: Methods and Protocols

  • Editors: Wendy A. Harwood

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8944-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8942-3Published: 20 November 2018

  • eBook ISBN: 978-1-4939-8944-7Published: 20 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 313

  • Number of Illustrations: 18 b/w illustrations, 95 illustrations in colour

  • Topics: Plant Breeding/Biotechnology, Agriculture

Publish with us

Policies and ethics

Back to top

Search

Navigation