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Immunological Tolerance

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Ashleigh S. Boyd

    1. Associate Professor in Stem Cell Biology and Regenerative Medicine, Research Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London (UCL), Royal Free Hospital, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1899)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Isolation of Stem Cells and Their Progeny for Immunological Tolerance Applications

    1. Front Matter

      Pages 1-1
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    2. Isolation and Characterisation of Human Adipose-Derived Stem Cells

      • Anna Wilson, Melisse Chee, Peter Butler, Ashleigh S. Boyd
      Pages 3-13

    3. Isolation of Murine Hematopoietic Stem Cells

      • Juan Bautista Menendez-Gonzalez, Lubaid Saleh, Gui Jie Feng, Neil P. Rodrigues
      Pages 15-23

    4. Generation of Antigen-Specific T Cells from Human Induced Pluripotent Stem Cells

      • Toshinobu Nishimura, Hiromitsu Nakauchi
      Pages 25-40

  3. Isolation of Regulatory Lymphocyte Populations

    1. Front Matter

      Pages 41-41
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    2. Isolation of Human Regulatory T Lymphocytes by Fluorescence-Activated Cell Sorting

      • Kate Milward, Joanna Hester, Kathryn J. Wood
      Pages 43-54

    3. Identification and Isolation of Regulatory B Cells in Mouse and Human

      • Madhvi Menon, Elizabeth C. Rosser, Claudia Mauri
      Pages 55-66

    4. An Innovative Standard Operation Procedure for Isolating GMP-Grade CD4+CD25+ T Cells from Non-Mobilized Leukapheresis

      • Wei Zhang, Suzanne M. Watt, David J. Roberts
      Pages 67-83

  4. Mechanisms and Induction of Immunological Tolerance

    1. Front Matter

      Pages 85-85
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    2. Measuring CTLA-4-Dependent Suppressive Function in Regulatory T Cells

      • Tie Zheng Hou, Omar S. Qureshi, David M. Sansom
      Pages 87-101

    3. Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation In Vitro in the Context of Costimulatory Molecule Blockade

      • Christine M. Barbon, Kenneth J. Janec, Rowan H. Kelner, James E. Norton, Eva C. Guinan
      Pages 103-118

    4. In Vitro Suppression of CD4+ T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cells

      • Katerina Vlachou, Panayotis Verginis
      Pages 119-128

    5. Disassembling and Reaggregating the Thymus: The Pros and Cons of Current Assays

      • Elia Piccinini, Paola Bonfanti
      Pages 129-142

    6. Isolation of Highly Viable Thymic Epithelial Cells for Use in In Vitro and In Vivo Experiments

      • Mi-Jeong Kim, Thomas Serwold
      Pages 143-156

  5. Transplantation Tolerance

    1. Front Matter

      Pages 157-157
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    2. Immune Tolerance and Rejection in Organ Transplantation

      • Jessica Stolp, Masaaki Zaitsu, Kathryn J. Wood
      Pages 159-180

    3. Method of Generating Tolerogenic Maturation-Resistant Dendritic Cells and Testing for Their Immune-Regulatory Functions In Vivo in the Context of Transplantation

      • Sherrie J. Divito, Adrian E. Morelli
      Pages 181-193

  6. The Breakdown of Immunological Tolerance in Disease

    1. Front Matter

      Pages 195-195
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    2. Luminex-Coupled EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Antigen-Specific T cells in Human Peripheral Blood

      • HuiJuan Lv, Khadir Raddassi, Myra A. Lipes
      Pages 197-210
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Keywords

About this book

This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Associate Professor in Stem Cell Biology and Regenerative Medicine, Research Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London (UCL), Royal Free Hospital, London, UK

    Ashleigh S. Boyd

Bibliographic Information

  • Book Title: Immunological Tolerance

  • Book Subtitle: Methods and Protocols

  • Editors: Ashleigh S. Boyd

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8938-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8936-2Published: 17 January 2019

  • eBook ISBN: 978-1-4939-8938-6Published: 16 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 240

  • Number of Illustrations: 7 b/w illustrations, 36 illustrations in colour

  • Topics: Immunology

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