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Cellular Senescence

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Marco Demaria

    1. ERIBA, University Medical Center Groningen, Groningen, The Netherlands

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1896)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Detecting Cellular Senescence in Reprogramming

    • Coralie Cazin, Mathieu von Joest, Han Li
    Pages 1-10

  3. DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA)

    • Alessandro Galbiati, Fabrizio d’Adda di Fagagna
    Pages 11-20

  4. Reactive Oxygen Species Detection in Senescent Cells

    • Stella Victorelli, João F. Passos
    Pages 21-29

  5. Cellular Identification and Quantification of Senescence-Associated β-Galactosidase Activity In Vivo

    • Bennett G. Childs, Tyler J. Bussian, Darren J. Baker
    Pages 31-38

  6. Relative Human Telomere Length Quantification by Real-Time PCR

    • A. Vasilishina, A. Kropotov, I. Spivak, A. Bernadotte
    Pages 39-44

  7. Assessing Functional Roles of the Senescence-Associated Secretory Phenotype (SASP)

    • Nicolas Malaquin, Véronique Tu, Francis Rodier
    Pages 45-55

  8. Measuring the Inflammasome in Oncogene-Induced Senescence

    • Irene Fernández-Duran, Núria Tarrats, Priya Hari, Juan Carlos Acosta
    Pages 57-70

  9. Alarmin Detection in Senescent Cells

    • Dong Eun Kim, Albert R. Davalos
    Pages 71-81

  10. IMR90 ER:RAS: A Cell Model of Oncogene-Induced Senescence

    • Andrew J. Innes, Jesús Gil
    Pages 83-92

  11. Genotoxic Stress-Induced Senescence

    • Dorothy N. Y. Fan, Clemens A. Schmitt
    Pages 93-105

  12. A Multiparametric Assay to Evaluate Senescent Cells

    • Hilah Gal, Ziv Porat, Valery Krizhanovsky
    Pages 107-117

  13. A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in Fluids

    • Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou et al.
    Pages 119-138

  14. Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry

    • Christopher D. Wiley, Sonnet Davis, Arvind Ramanathan
    Pages 139-147

  15. Quantification of Autophagy During Senescence

    • Joon Tae Park, Young-Sam Lee, Sang Chul Park
    Pages 149-157

  16. Quantification of Aneuploidy in Mammalian Systems

    • Hilda van den Bos, Bjorn Bakker, Aaron Taudt, Victor Guryev, Maria Colomé-Tatché, Peter M. Lansdorp et al.
    Pages 159-190

  17. Identification of Genomic Alterations Through Multilevel DNA Structural Analysis

    • Ryan K. Shultzaberger, John Dresios
    Pages 191-201

  18. Mouse Models of Accelerated Cellular Senescence

    • Matthew J. Yousefzadeh, Kendra I. Melos, Luise Angelini, Christin E. Burd, Paul D. Robbins, Laura J. Niedernhofer
    Pages 203-230

  19. Methods to Quantify the NF-κB Pathway During Senescence

    • Lei Zhang, Jing Zhao, Aditi Gurkar, Laura J. Niedernhofer, Paul D. Robbins
    Pages 231-250

  20. Correction to: A Novel Quantitative Method for the Detection of Lipofuscin, the Main By-Product of Cellular Senescence, in Fluids

    • Sophia V. Rizou, Konstantinos Evangelou, Vassilios Myrianthopoulos, Iordanis Mourouzis, Sophia Havaki, Aikaterini Athanasiou et al.
    Pages C1-C2
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Keywords

About this book

This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • ERIBA, University Medical Center Groningen, Groningen, The Netherlands

    Marco Demaria

Bibliographic Information

  • Book Title: Cellular Senescence

  • Book Subtitle: Methods and Protocols

  • Editors: Marco Demaria

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8931-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8930-0Published: 24 November 2018

  • eBook ISBN: 978-1-4939-8931-7Published: 24 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 253

  • Number of Illustrations: 7 b/w illustrations, 39 illustrations in colour

  • Topics: Cell Biology

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