Suicide Gene Therapy

1. Front Matter

   Pages i-xii

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2. Origins of Suicide Gene Therapy

   • Nejat Düzgüneş

   Pages 1-9

3. Cancer Suicide Gene Therapy with TK.007

   • Jubayer A. Hossain, Kristoffer Riecken, Hrvoje Miletic, Boris Fehse

   Pages 11-26

4. Osteonectin Promoter-Mediated Suicide Gene Therapy of Prostate Cancer

   • Wan-Chi Hsiao, Shian-Ying Sung, Leland W. K. Chung, Chia-Ling Hsieh

   Pages 27-42

5. Suicide Gene Therapy for Oral Squamous Cell Carcinoma

   • Henrique Faneca, Nejat Düzgüneş, Maria C. Pedroso de Lima

   Pages 43-55

6. Generation of Suicide Gene-Modified Chimeric Antigen Receptor-Redirected T-Cells for Cancer Immunotherapy

   • Kentaro Minagawa, Mustafa Al-Obaidi, Antonio Di Stasi

   Pages 57-73

7. Mesenchymal Stem Cell Exosome-Mediated Prodrug Gene Therapy for Cancer

   • Cestmir Altaner, Ursula Altanerova

   Pages 75-85

8. Extracellular Vesicles as Carriers of Suicide mRNA and/or Protein in Cancer Therapy

   • Erdogan Pekcan Erkan, Nurten Saydam, Clark C. Chen, Okay Saydam

   Pages 87-96

9. Production of Viral Vectors with Suicide Genes by Utilizing the Intron-Splicing Mechanism of Insect Cells

   • Haifeng Chen

   Pages 97-109

10. Resveratrol-Responsive CArG Elements from the Egr-1 Promoter for the Induction of GADD45α to Arrest the G2/M Transition

    • Qiwen Shi, Deepak Bhatia

    Pages 111-122

11. Noninvasive Monitoring of Suicide Gene Therapy by Using Multimodal Molecular Imaging

    • Bryan Holvoet, Cindy Leten, Christophe M. Deroose, Uwe Himmelreich

    Pages 123-134

12. In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector

    • Alexey A. Komissarov, Sergey V. Kostrov, Ilya V. Demidyuk

    Pages 135-147

13. Engineered Thymidine-Active Deoxycytidine Kinase for Bystander Killing of Malignant Cells

    • Anton Neschadim, Jeffrey A. Medin

    Pages 149-163

14. Photochemical Internalization Enhanced Nonviral Suicide Gene Therapy

    • Chung-Ho Sun, Kristian Berg, Henry Hirschberg

    Pages 165-176

15. Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro

    • Nejat Düzgüneş, Jennifer Cheung, Krystyna Konopka

    Pages 177-184

16. Back Matter

    Pages 185-190

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This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  

Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.

• Cancer treatment
• Therapeutic genes
• Tumors
• Cell therapy
• Bone marrow mesenchymal cells
• gene therapy

• Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific, San Francisco, USA

  Nejat Düzgüneş

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