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The Hippo Pathway

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Alexander Hergovich

    1. Cancer Institute, University College London, London, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1893)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. The Hippo Pathway: Drosophila Genetics Lead the Way

    1. Front Matter

      Pages 1-1
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    2. The Power of Drosophila Genetics: The Discovery of the Hippo Pathway

      • Rewatee Gokhale, Cathie M. Pfleger
      Pages 3-26

    3. Drosophila Genetics: The Power of Genetic Mosaic Approaches

      • Mardelle Atkins
      Pages 27-42

    4. Drosophila Genetics: Analysis of Tissue Growth in Adult Tissues

      • Alexander D. Fulford, Paulo S. Ribeiro
      Pages 43-51

    5. Live Imaging of Hippo Pathway Components in Drosophila Imaginal Discs

      • Jiajie Xu, Ting Su, Sherzod A. Tokamov, Richard G. Fehon
      Pages 53-59

    6. Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence

      • Cordelia Rauskolb, Kenneth D. Irvine
      Pages 61-73

    7. Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions

      • Yurika Matsui, Zhi-Chun Lai
      Pages 75-85

  3. Molecular and Cell Biology Studies of the Hippo Pathway

    1. Front Matter

      Pages 87-87
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    2. Immunohistochemistry to Study YAP in Human Tissue Samples

      • Franziska Haderk, Victor Olivas, Trever G. Bivona
      Pages 89-95

    3. Immunofluorescence Study of Endogenous YAP in Mammalian Cells

      • Valentina Rausch, Carsten G. Hansen
      Pages 97-106

    4. Immunofluorescence Microscopy to Study Endogenous TAZ in Mammalian Cells

      • Nathan M. Kingston, Andrew M. Tilston-Lunel, Julia Hicks-Berthet, Xaralabos Varelas
      Pages 107-113

    5. Nuclear/Cytoplasmic Fractionation to Study Hippo Effectors

      • Maria Chatzifrangkeskou, Eric O’Neill
      Pages 115-119

    6. Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells

      • Sirio Dupont
      Pages 121-135

    7. Quantitative Real-Time PCR to Measure YAP/TAZ Activity in Human Cells

      • Xiaolei Cao, Bin Zhao
      Pages 137-152

    8. HTRF® Total and Phospho-YAP (Ser127) Cellular Assays

      • Diana Zindel, Claire Vol, Odile Lecha, Isabelle Bequignon, Merve Bilgic, Marion Vereecke et al.
      Pages 153-166

    9. Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos and Human Spheroids

      • Yoichi Asaoka, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp Heisenberg, Makoto Furutani-Seiki
      Pages 167-181

    10. Regulation of YAP/TAZ Activity by Mechanical Cues: An Experimental Overview

      • Sirio Dupont
      Pages 183-202

    11. CRISPR-Mediated Approaches to Regulate YAP/TAZ Levels

      • Ryan J. Quinton, Neil J. Ganem
      Pages 203-214

    12. Hippo Pathway Regulation by Tyrosine Kinases

      • Nina Reuven, Matan Shanzer, Yosef Shaul
      Pages 215-236
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Keywords

About this book

This detailed book covers numerous expert methodologies to examine Hippo signaling on the structural, molecular, cellular, and organismal level. Beginning with a section on Drosophila genetics, the volume continues with parts exploring molecular and cell biological studies of the Hippo pathway, the use of structural biology and biochemistry, and a brief look at the Hippo pathway in mouse models. Written for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  

Comprehensive and practical, The Hippo Pathway: Methods and Protocols equips newcomers and specialists alike with key methodologies to accurately define the status of Hippo signaling in their experimental settings.

Editors and Affiliations

  • Cancer Institute, University College London, London, UK

    Alexander Hergovich

Bibliographic Information

  • Book Title: The Hippo Pathway

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander Hergovich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8910-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8909-6Published: 19 December 2018

  • eBook ISBN: 978-1-4939-8910-2Published: 18 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 374

  • Number of Illustrations: 15 b/w illustrations, 57 illustrations in colour

  • Topics: Biochemistry, general

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