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Lactic Acid Bacteria

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Makoto Kanauchi

    1. Department of Food Management, Miyagi University, Sendai, Japan

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1887)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
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  3. Applications of Food Industries of LABs

    1. Front Matter

      Pages 43-43
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    2. Yogurt Production

      • Seiji Nagaoka
      Pages 45-54

    3. Purification, Rheological Characterization, and Visualization of Viscous, Neutral, Hetero-exopolysaccharide Produced by Lactic Acid Bacteria

      • S. Ikeda, D. Kondoh, N. P. D. Aryantini, T. Urashima, K. Fukuda
      Pages 55-65

    4. Structural Analysis of Exopolysaccharides from Lactic Acid Bacteria

      • Gerrit J. Gerwig
      Pages 67-84

    5. Preparation of Exopolysaccharide Synthesized by Lactic Acid Bacteria

      • Junko Nishimura
      Pages 85-94

    6. PCR Analysis Methods for Detection and Identification of Beer-Spoilage Lactic Acid Bacteria

      • S. Asano, M. Shimokawa, K. Suzuki
      Pages 95-107

    7. Isolation of Lactic Acid Bacteria Eliminating Trimethylamine (TMA) for Application to Fishery Processing

      • Satoshi Mohri, Makoto Kanauchi
      Pages 109-117

    8. Screening the Lactic Acid Bacteria converting Hydroxy Fatty Acid from Unsaturated Fatty Acid

      • Makoto Kanauchi
      Pages 119-127

  4. Healthy Functions of LABs

    1. Front Matter

      Pages 129-129
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    2. Screening and Characterization of Immunobiotic Lactic Acid Bacteria with Porcine Immunoassay Systems

      • Valeria Garcia-Castillo, Leonardo Albarracin, Haruki Kitazawa, Julio Villena
      Pages 131-144

    3. Biosorption of Heavy Metals by Lactic Acid Bacteria for Detoxification

      • Hideki Kinoshita
      Pages 145-157

    4. Adhesion of Lactobacillus to Intestinal Mucin

      • Keita Nishiyama, Takao Mukai
      Pages 159-166

    5. Eliminating Lipopolysaccharide (LPS) Using Lactic Acid Bacteria (LAB) and a Fraction of its LPS-Elimination Protein

      • Makoto Kanauchi, Ayaka Kondo, Kyoko Asami
      Pages 167-174

    6. Cloning and Expression of Lipopolysaccharide Elimination Protein (LEP) in Lactic Acid Bacteria

      • Kyoko Asami, Makoto Kanauchi
      Pages 175-187
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Keywords

About this book

This detailed book provides a collection of protocols for numerous experimental approaches perfected by the authors for lactic acid bacteria (LAB) research.  Split in to three parts, the volume delves into the identification and metabolism of LABs, the applications of the bacteria for the food industry, as well as healthy functions of LAB.  Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Lactic Acid Bacteria: Methods and Protocols serves as an ideal inspiration for many research efforts in the domains of food science and health science.

Editors and Affiliations

  • Department of Food Management, Miyagi University, Sendai, Japan

    Makoto Kanauchi

Bibliographic Information

  • Book Title: Lactic Acid Bacteria

  • Book Subtitle: Methods and Protocols

  • Editors: Makoto Kanauchi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8907-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8906-5Published: 01 December 2018

  • eBook ISBN: 978-1-4939-8907-2Published: 30 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 194

  • Number of Illustrations: 48 b/w illustrations, 10 illustrations in colour

  • Topics: Microbiology

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