Skip to main content
SpringerLink
Log in

Atomic Force Microscopy

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Nuno C. Santos

    1. Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Filomena A. Carvalho

    1. Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge lab techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1886)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Imaging

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Artifacts and Practical Issues in Atomic Force Microscopy

      • Peter Eaton, Krystallenia Batziou
      Pages 3-28

    3. Quantitative Analysis of Structure and Dynamics in AFM Images of Lipid Membranes

      • Simon D. Connell, George R. Heath, James A. Goodchild
      Pages 29-44

    4. Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy

      • Hussein Nasrallah, Anthony Vial, Nicolas Pocholle, Jérémy Soulier, Luca Costa, Cédric Godefroy et al.
      Pages 45-59

    5. Investigating the Nanodomain Organization of Rhodopsin in Native Membranes by Atomic Force Microscopy

      • Subhadip Senapati, Paul S.-H. Park
      Pages 61-74

    6. Analysis of DNA–Protein Complexes by Atomic Force Microscopy Imaging: The Case of TRF2–Telomeric DNA Wrapping

      • Sabrina Pisano, Eric Gilson
      Pages 75-97

    7. Cell Topography and Its Quantitative Imaging by AFM

      • Jiang Pi, Jiye Cai
      Pages 99-113

  3. Single-Molecule Force Spectroscopy

    1. Front Matter

      Pages 115-115
      Download chapter PDF

    2. Functionalization of AFM Tips and Supports for Molecular Recognition Force Spectroscopy and Recognition Imaging

      • A. Ebner, L. Wildling, H. J. Gruber
      Pages 117-151

    3. Ligand-Receptor Binding on Cell Membrane: Dynamic Force Spectroscopy Applications

      • Jianli Liu, Wenhui Li, Xuejie Zhang, Yan Feng, Xiaohong Fang
      Pages 153-162

    4. Single-Molecule Force Spectroscopy: Experiments, Analysis, and Simulations

      • Fidan Sumbul, Felix Rico
      Pages 163-189

    5. AFM to Study Pore-Forming Proteins

      • Joseph D. Unsay, Ana J. García-Sáez
      Pages 191-202

    6. Imaging and Manipulation of Extracellular Traps by Atomic Force Microscopy

      • Ricardo H. Pires, Mihaela Delcea, Stephan B. Felix
      Pages 203-217

  4. Studies of Bacteria and Virus in AFM

    1. Front Matter

      Pages 219-219
      Download chapter PDF

    2. Investigation of Bacterial Curli Production and Adhesion Using AFM

      • Yoo Jin Oh, Peter Hinterdorfer
      Pages 221-231

    3. Antimicrobial Peptides: Effect on Bacterial Cells

      • Marco M. Domingues, Mário R. Felício, Sónia Gonçalves
      Pages 233-242

    4. AFM Nanoindentation Experiments on Protein Shells: A Protocol

      • Yukun Guo, Wouter H. Roos
      Pages 243-257

    5. Structural and Mechanical Characterization of Viruses with AFM

      • Álvaro Ortega-Esteban, Natália Martín-González, Francisco Moreno-Madrid, Aida Llauró, Mercedes Hernando-Pérez, Cármen San MartÚn et al.
      Pages 259-278

  5. AFM Elasticity Studies

    1. Front Matter

      Pages 279-279
      Download chapter PDF
Back to top

Keywords

About this book

This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.

Editors and Affiliations

  • Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal

    Nuno C. Santos, Filomena A. Carvalho

Bibliographic Information

  • Book Title: Atomic Force Microscopy

  • Book Subtitle: Methods and Protocols

  • Editors: Nuno C. Santos, Filomena A. Carvalho

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8894-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8893-8Published: 30 October 2018

  • Softcover ISBN: 978-1-4939-9407-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8894-5Published: 29 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 372

  • Number of Illustrations: 16 b/w illustrations, 87 illustrations in colour

  • Topics: Biological Microscopy

Publish with us

Policies and ethics

Back to top

Search

Navigation