Skip to main content
  • Book
  • © 2019

Skin Stem Cells

Methods and Protocols

Editors:

  • Includes fully revised and all new cutting-edge methods and protocols for the study of skin stem cell research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1879)

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (33 protocols)

  1. Front Matter

    Pages i-xv
  2. In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging

    • Olivia Farrelly, Paola Kuri, Panteleimon Rompolas
    Pages 1-14
  3. Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells

    • Daniel Haensel, Melissa A. McNeil, Xing Dai
    Pages 15-29
  4. Isolation and Characterization of Cutaneous Epithelial Stem Cells

    • Stephanie R. Gillespie, David M. Owens
    Pages 87-99
  5. Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin

    • Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, Alexandra P. Marques
    Pages 101-110
  6. Isolation and Cultivation of Epidermal (Stem) Cells

    • Xusheng Wang, Shiyang Dong, Yaojiong Wu
    Pages 133-138
  7. One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen

    • Hua Qian, Xue Leng, Jie Wen, Qian Zhou, Xin Xu, Xunwei Wu
    Pages 139-148
  8. Isolation and Cultivation of Skin-Derived Precursors

    • Xiaoxiao Wang, Shiyang Dong, Yaojiong Wu
    Pages 149-152
  9. Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

    • Petek Korkusuz, Sevil Köse, Nilgün Yersal, Selin Önen
    Pages 153-163
  10. Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair

    • Mahmut Parmaksiz, AyÅŸe Eser Elçin, YaÅŸar Murat Elçin
    Pages 175-185
  11. Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

    • Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, Amir Bajouri
    Pages 201-210
  12. Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration

    • Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, Amir Bajouri
    Pages 211-219
  13. Skin Wound Healing: Refractory Wounds and Novel Solutions

    • Gabriel M. Virador, Lola de Marcos, Victoria M. Virador
    Pages 221-241

About this book

This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells.

Editors and Affiliations

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Skin Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8870-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8869-3Published: 30 January 2019

  • eBook ISBN: 978-1-4939-8870-9Published: 29 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 463

  • Number of Illustrations: 29 b/w illustrations, 76 illustrations in colour

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access