Skip to main content
SpringerLink
Log in
Book cover

Protein Misfolding Diseases

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Cláudio M. Gomes

    1. BioISI – Biosystems and Integrative Sciences Institute, Faculty of Sciences University of Lisbon, Lisbon, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of protein folding diseases
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1873)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Protein Biophysics Assays

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation

      • Joana S. Cristóvão, Bárbara J. Henriques, Cláudio M. Gomes
      Pages 3-18

    3. Ultrasensitive RT-QuIC Seed Amplification Assays for Disease-Associated Tau, α-Synuclein, and Prion Aggregates

      • Eri Saijo, Bradley R. Groveman, Allison Kraus, Michael Metrick, Christina D. Orrù, Andrew G. Hughson et al.
      Pages 19-37

    4. Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides

      • Ravit Malishev, Sofiya Kolusheva, Raz Jelinek
      Pages 39-51

    5. Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis

      • Naoto Soya, Ariel Roldan, Gergely L. Lukacs
      Pages 53-67

    6. A Multipronged Method for Unveiling Subtle Structural–Functional Defects of Mutant Chaperone Molecules Causing Human Chaperonopathies

      • Donatella Bulone, Pier Luigi San Biagio, Tatiana Quiñones-Ruiz, Manuel Rosario-Alomar, Igor K. Lednev, Frank T. Robb et al.
      Pages 69-92

    7. High-Throughput Microplate-Based Fluorescence Assays for Studying Stochastic Aggregation of Superoxide Dismutase-1

      • Alireza Abdolvahabi, Sanaz Rasouli, Corbin M. Croom, Devon L. Plewman
      Pages 93-108

    8. Methods for Structural Analysis of Amyloid Fibrils in Misfolding Diseases

      • Devkee M. Vadukul, Youssra K. Al-Hilaly, Louise C. Serpell
      Pages 109-122

    9. Assays for Light Chain Amyloidosis Formation and Cytotoxicity

      • Luis M. Blancas-Mejia, Pinaki Misra, Christopher J. Dick, Marta Marin-Argany, Keely R. Redhage, Shawna A. Cooper et al.
      Pages 123-153

  3. Cellular and Proteostasis Assays

    1. Front Matter

      Pages 155-155
      Download chapter PDF

    2. Monitoring Aggregate Clearance and Formation in Cell-Based Assays

      • Evelien Eenjes, Young Joo Yang-Klingler, Ai Yamamoto
      Pages 157-169

    3. Monitoring Proteome Stress in Live Cells Using HaloTag-Based Fluorogenic Sensor

      • Yu Liu, Matthew Fares, Xin Zhang
      Pages 171-182

    4. Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation

      • Vibha Prasad, Aaron Voigt
      Pages 183-193

    5. Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry

      • Salvador Ventura, Susanna Navarro
      Pages 195-212

    6. Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells

      • Edward Pokrishevsky, Jeremy Nan, Neil R. Cashman
      Pages 213-224

    7. A Cell Model for HSP60 Deficiencies: Modeling Different Levels of Chaperonopathies Leading to Oxidative Stress and Mitochondrial Dysfunction

      • Cagla Cömert, Paula Fernandez-Guerra, Peter Bross
      Pages 225-239

    8. Superresolution Fluorescence Imaging of Mutant Huntingtin Aggregation in Cells

      • Steffen J. Sahl, Willianne I. M. Vonk
      Pages 241-251

  4. Protein Folding Recovery and Correction Assays

    1. Front Matter

      Pages 253-253
      Download chapter PDF

    2. Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry

      • Tânia G. Lucas, Cláudio M. Gomes, Bárbara J. Henriques
      Pages 255-264
Back to top

Keywords

About this book

This detailed book gathers a broad collection of experimental approaches to assist researchers in setting up different methods to investigate protein conformational disorders. Beginning with a section on assays focusing on biophysical approaches to study protein (mis)folding, the volume continues with sections on cellular and proteostasis assays as well as assays for protein folding correction and recovery, combining methods such as thermal shift assays, in silico improvement of protein solubility, and compound screening, an important area of research as it may open avenues for therapeutic strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Protein Misfolding Diseases:Methods and Protocols serves as an ideal guide for researchers seeking to advance our knowledge of protein conformational disorders.

Editors and Affiliations

  • BioISI – Biosystems and Integrative Sciences Institute, Faculty of Sciences University of Lisbon, Lisbon, Portugal

    Cláudio M. Gomes

Bibliographic Information

  • Book Title: Protein Misfolding Diseases

  • Book Subtitle: Methods and Protocols

  • Editors: Cláudio M. Gomes

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8820-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8819-8Published: 19 October 2018

  • Softcover ISBN: 978-1-4939-9396-3Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8820-4Published: 19 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 338

  • Number of Illustrations: 19 b/w illustrations, 58 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation