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  • © 2019

Functional Proteomics

Methods and Protocols

  • Includes cutting-edge techniques for the study of functional proteomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1871)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xii
  2. Making the Case for Functional Proteomics

    • Ray C. Perkins
    Pages 1-40
  3. A Protein Decomplexation Strategy in Snake Venom Proteomics

    • Choo Hock Tan, Kae Yi Tan, Nget Hong Tan
    Pages 83-92
  4. A Systematic Analysis Workflow for High-Density Customized Protein Microarrays in Biomarker Screening

    • Rodrigo García-Valiente, Jonatan Fernández-García, Javier Carabias-Sánchez, Alicia Landeira-Viñuela, Rafael Góngora, María Gonzalez-Gonzalez et al.
    Pages 107-122
  5. Metaproteomics Study of the Gut Microbiome

    • Lisa A. Lai, Zachary Tong, Ru Chen, Sheng Pan
    Pages 123-132
  6. Double One-Dimensional Electrophoresis (D1-DE) Adapted for Immunoproteomics

    • Youcef Shahali, Hélène Sénéchal, Pascal Poncet
    Pages 133-141
  7. BioID: A Proximity-Dependent Labeling Approach in Proteomics Study

    • Peipei Li, Yuan Meng, Li Wang, Li-jun Di
    Pages 143-151
  8. Proteomic Detection of Carbohydrate-Active Enzymes (CAZymes) in Microbial Secretomes

    • Tina R. Tuveng, Vincent G. H. Eijsink, Magnus Ø. Arntzen
    Pages 159-177
  9. Functional Proteomic Analysis to Characterize Signaling Crosstalk

    • Sneha M. Pinto, Yashwanth Subbannayya, T. S. Keshava Prasad
    Pages 197-224
  10. Targeted Proteomics

    • Yun Chen, Liang Liu
    Pages 265-277
  11. Metabolomic Investigation of Staphylococcus aureus Antibiotic Susceptibility by Liquid Chromatography Coupled to High-Resolution Mass Spectrometry

    • Sandrine Aros-Calt, Florence A. Castelli, Patricia Lamourette, Gaspard Gervasi, Christophe Junot, Bruno H. Muller et al.
    Pages 279-293
  12. Nuts and Bolts of Protein Quantification by Online Trypsin Digestion Coupled LC-MS/MS Analysis

    • Christopher A. Toth, Zsuzsanna Kuklenyik, John R. Barr
    Pages 295-311

About this book

This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use this mechanism in some capacity to carry out their unique role in cellular regulation. By comparing functional proteomes, this presents a bridge to other levels of system biology research including genomics and metabolomics in order to provide readers with a relatively complete picture for how one might study the biological system of their interest. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritativeand cutting-edge, Functional Proteomics: Methods and Protocols collects these novel technologies in the hope that new frontiers in biological research will be created, important drug targets can be identified, and clinically validated biomarkers and diagnostic tests can be further developed.


Editors and Affiliations

  • Array Bridge Inc., St. Louis, USA

    Xing Wang

  • Biotech Support Group LLC, Monmouth Junction, USA

    Matthew Kuruc

Bibliographic Information

  • Book Title: Functional Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Xing Wang, Matthew Kuruc

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8814-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8813-6Published: 01 October 2018

  • Softcover ISBN: 978-1-4939-9395-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8814-3Published: 01 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 477

  • Number of Illustrations: 26 b/w illustrations, 63 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access