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Brain Tumor Stem Cells

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Sheila K. Singh

    1. Stem Cell and Cancer Research Institute, McMaster University, Hamilton, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Chitra Venugopal

    1. Stem Cell and Cancer Research Institute, McMaster University, Hamilton, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of brain tumor stem cell research
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1869)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Introduction to Brain Tumor Stem Cells

    • Nicolas Yelle, David Bakhshinyan, Chitra Venugopal, Sheila K. Singh
    Pages 1-9

  3. Isolation and Culture of Glioblastoma Brain Tumor Stem Cells

    • Charles Chesnelong, Ian Restall, Samuel Weiss
    Pages 11-21

  4. Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines

    • Sara Badodi, Silvia Marino, Loredana Guglielmi
    Pages 23-36

  5. Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors

    • Vijay Ramaswamy, Michael D. Taylor
    Pages 37-56

  6. Detecting Stem Cell Marker Expression Using the NanoString nCounter System

    • Scott Ryall, Anthony Arnoldo, Javal Sheth, Sheila K. Singh, Cynthia Hawkins
    Pages 57-67

  7. Flow Cytometric Analysis of Brain Tumor Stem Cells

    • Minomi K. Subapanditha, Ashley A. Adile, Chitra Venugopal, Sheila K. Singh
    Pages 69-77

  8. In Vitro Self-Renewal Assays for Brain Tumor Stem Cells

    • Mathieu Seyfrid, David Bobrowski, David Bakhshinyan, Nazanin Tatari, Chitra Venugopal, Sheila K. Singh
    Pages 79-84

  9. Differentiation of Brain Tumor Initiating Cells

    • Michelle M. Kameda-Smith, Minomi K. Subapanditha, Sabra K. Salim, Chitra Venugopal, Sheila K. Singh
    Pages 85-91

  10. The Study of Brain Tumor Stem Cell Migration

    • Montserrat Lara-Velazquez, Rawan Al-kharboosh, Luis Prieto, Paula Schiapparelli, Alfredo Quiñones-Hinojosa
    Pages 93-104

  11. The Study of Brain Tumor Stem Cell Invasion

    • Rawan Al-kharboosh, Montserrat Lara-Velazquez, Luis Prieto, Rachel Sarabia-Estrada, Alfredo Quiñones-Hinojosa
    Pages 105-116

  12. Cell Cycle Dynamics in Glioma Cancer Stem Cells

    • Ingrid Qemo, Lisa A. Porter
    Pages 117-126

  13. Embryonic Stem Cell Models of Human Brain Tumors

    • Ludivine Coudière Morrison, Nazanin Tatari, Tamra E. Werbowetski-Ogilvie
    Pages 127-142

  14. Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory

    • Hunter McColl, Jamie L. Zagozewski, David D. Eisenstat
    Pages 143-153

  15. EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF

    • Amy X. Hu, Jarrett J. Adams, Parvez Vora, Maleeha Qazi, Sheila K. Singh, Jason Moffat et al.
    Pages 155-168

  16. Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells

    • Michael Aregger, Megha Chandrashekhar, Amy Hin Yan Tong, Katherine Chan, Jason Moffat
    Pages 169-188

  17. In Vitro Assays for Screening Small Molecules

    • Ashley A. Adile, David Bakhshinyan, Chitra Venugopal, Sheila K. Singh
    Pages 189-196

  18. Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma

    • Elena Binda, Alberto Visioli, Nadia Trivieri, Angelo Luigi Vescovi
    Pages 197-205

  19. Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci

    • Jon D. Larson, Suzanne J. Baker
    Pages 207-230

  20. In Vivo Murine Models of Brain Metastasis

    • Mohini Singh, Neil Savage, Sheila K. Singh
    Pages 231-238
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Keywords

About this book

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocolsserves as an ideal guide for researchers seeking to better understand the complexities of brain cancer.

Reviews

“Sheila K. Singh and Chitra Venugopal have developed a concise, systematic, and practical book; an ideal read for brain cancer researchers. … this book is recommended for anyone who strives for proficiency in biophysiology of cancer stem cells and hopes to discover novel therapeutic and treatment strategies for patients affected by devastating CNS disease.” (Angela M. Bohnen, Neurosurgery, Vol. 85 (4), October, 2019)

Editors and Affiliations

  • Stem Cell and Cancer Research Institute, McMaster University, Hamilton, Canada

    Sheila K. Singh, Chitra Venugopal

Bibliographic Information

  • Book Title: Brain Tumor Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Sheila K. Singh, Chitra Venugopal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8805-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8804-4Published: 16 October 2018

  • Softcover ISBN: 978-1-4939-9394-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8805-1Published: 15 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 254

  • Number of Illustrations: 6 b/w illustrations, 34 illustrations in colour

  • Topics: Cancer Research, Neurosciences

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