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Rheumatoid Arthritis

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Shuang Liu

    1. Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1868)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-ix
    Download chapter PDF

  2. Animal Models

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Collagen-Induced Arthritis Models

      • Maya Miyoshi, Shuang Liu
      Pages 3-7

    3. Human Xenograft Model

      • Shuang Liu
      Pages 9-15

    4. Long-Term Constant Subcutaneous Drug Administration

      • Shuang Liu, Maya Miyoshi
      Pages 17-22

    5. Clinical Scoring of Disease Activity in Animal Models

      • Maya Miyoshi, Shuang Liu
      Pages 23-28

    6. Histological Analysis of Arthritic Joints

      • Takeshi Kiyoi
      Pages 29-39

    7. Preparation of Joint Extracts

      • Shuang Liu, Erika Takemasa
      Pages 41-45

  3. Therapeutic Approach

    1. Front Matter

      Pages 47-47
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    2. Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System

      • Wei Zhou, Hiroyuki Takeda
      Pages 49-67

    3. Reconstruction of Protein/Liposome Complex

      • Yasuyuki Suzuki
      Pages 69-77

    4. Production of Neutralizing Antibody

      • Erika Takemasa, Shuang Liu, Hitoshi Hasegawa
      Pages 79-92

    5. Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen

      • Hiroyuki Takeda
      Pages 93-112

    6. Generation of Specific Aptamers

      • Shuang Liu, Yasuyuki Suzuki, Makoto Inui
      Pages 113-121

    7. Production of Lentiviral Particles

      • Shuang Liu
      Pages 123-128

    8. RNA Interference Ex Vivo

      • Shuang Liu
      Pages 129-135

    9. Lentiviral-Mediated Systemic RNA Interference In Vivo

      • Shuang Liu
      Pages 137-144

    10. Mesenchymal Stem Cell Engineering

      • Shuang Liu
      Pages 145-150

  4. Evaluation of Immunological Status

    1. Front Matter

      Pages 151-151
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    2. Screening of Ca2+ Influx in Lymphocytes

      • Erika Takemasa, Shuang Liu
      Pages 153-159
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Keywords

About this book

This volume provides a summary of the standard laboratory protocols and methodology commonly used in basic and translational studies in the field of rheumatoid arthritis (RA) treatment. Chapters detail including basic RA models, evaluation of disease activity and immunological status, systemic drug delivery, and new research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Rheumatoid Arthritis: Methods and Protocols aims to be a basic manual for clinical researchers who are just getting started in the field of intervention study.

Editors and Affiliations

  • Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Japan

    Shuang Liu

About the editor



Bibliographic Information

  • Book Title: Rheumatoid Arthritis

  • Book Subtitle: Methods and Protocols

  • Editors: Shuang Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8802-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8801-3Published: 23 September 2018

  • Softcover ISBN: 978-1-4939-9393-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8802-0Published: 22 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: IX, 253

  • Number of Illustrations: 25 b/w illustrations, 34 illustrations in colour

  • Topics: Molecular Medicine

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